The antiserum was produced against synthesized peptide derived from human AMPK beta1 around the phosphorylation site of Ser181.
Immunogen Range
147-196
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
AMPK beta1 (Phospho-Ser181) Antibody detects endogenous levels of AMPK beta1 only when phosphorylated at Ser181.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PRKAB1
Database Links
Gene ID: 5564 Swiss-Prot #: Q9Y478
OMIM #: 602740 Unigene #: Hs.6061
Target Modification
Phospho
Modification Sites
H:S182 M:S182 R:S182
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using AMPK beta1 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COLO205 cells and HUVEC cells, using AMPK beta1 (Phospho-Ser181) Antibody. The lane on the right is blocked with the phospho peptide.