The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
SGK (Phospho-Ser422) Antibody
Synonyms
SGK1, SERUM/GLUCOCORTICOID-REGULATED KINASE
Immunogen
The antiserum was produced against synthesized peptide derived from human SGK around the phosphorylation site of Ser422.
Immunogen Range
381-430
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
SGK (Phospho-Ser422) Antibody detects endogenous levels of SGK only when phosphorylated at Ser422.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
SGK1
Database Links
Gene ID: 6446 Swiss-Prot #: O00141
OMIM #: 602958 Unigene #: Hs.510078
Target Modification
Phospho
Modification Sites
H:S422 M:S422 R:S421
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HeLa cells treated with Insulin 0.01U/ml 15', using SGK (Phospho-Ser422) Antibody. The lane on the right is blocked with the phospho peptide.