c-PLA2 (Phospho-Ser505) Antibody detects endogenous levels of c-PLA2 only when phosphorylated at Ser505.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PLA2G4A
Database Links
Gene ID: 5321 Swiss-Prot #: P47712
OMIM #: 600522 Unigene #: Hs.497200
Target Modification
Phospho
Modification Sites
H:S505 M:S505 R:S505
Application Images
Image 1
Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using c-PLA2 (Phospho-Ser505) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using c-PLA2 (Phospho-Ser505) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml 30', using c-PLA2 (Phospho-Ser505) Antibody. The lane on the right is blocked with the phospho peptide.