Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | CDC25B(Phospho-Ser323)Antibody |
Synonyms | CDC25HU2;CDC25M2;DualspecificityphosphataseCdc25B;M-phaseinducerphosphatase2;MPIP2 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanCDC25BaroundthephosphorylationsiteofSer323. |
ImmunogenRange | 289-338 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000ELISA:1:20000 |
Specificity | CDC25B(Phospho-Ser323)AntibodydetectsendogenouslevelsofCDC25BonlywhenphosphorylatedatSer323. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | CDC25A |
DatabaseLinks | GeneID: 994 Swiss-Prot#: P30305 OMIM#: 116949 Unigene#: Hs.437705 |
TargetModification | Phospho |
ModificationSites | H:S323M:S321R:S319 |
ApplicationImages |
Image1 | WesternblotanalysisoflysatesfromNIH/3T3cellstreatedwithPMA125ng/ml30",usingCDC25B(Phospho-Ser323)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |