The antiserum was produced against synthesized peptide derived from human BIK around the phosphorylation site of Thr33.
Immunogen Range
18-67
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
BIK (Phospho-Thr33) Antibody detects endogenous levels of BIK only when phosphorylated at Thr33.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BIK
Database Links
Gene ID: 638 Swiss-Prot #: Q13323
OMIM #: 603392 Unigene #: Hs.475055
Target Modification
Phospho
Modification Sites
H:T33
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using BIK (Phospho-Thr33) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from K562 cells, using BIK (Phospho-Thr33) Antibody. The lane on the right is blocked with the phospho peptide.