The antiserum was produced against synthesized peptide derived from human Trk B around the phosphorylation site of Tyr515.
Immunogen Range
481-530
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
Trk B (Phospho-Tyr515) Antibody detects endogenous levels of Trk B only when phosphorylated at Tyr515.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NTRK2
Database Links
Gene ID: 4915 Swiss-Prot #: Q16620
OMIM #: 600456 Unigene #: Hs.494312/Hs.712776
Target Modification
Phospho
Modification Sites
H:Y516 M:Y515 R:Y515
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using Trk B (Phospho-Tyr515) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from NIH/3T3 cells, using Trk B (Phospho-Tyr515) Antibody. The lane on the right is blocked with the phospho peptide.