The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Smad2 (Phospho-Ser467) Antibody
Synonyms
JV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2; Mothers against decapentaplegic homolog 2; Smad 2
Immunogen
The antiserum was produced against synthesized peptide derived from human Smad2 around the phosphorylation site of Ser467.
Immunogen Range
418-467
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
Smad2 (Phospho-Ser467) Antibody detects endogenous levels of Smad2 only when phosphorylated at Ser467.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
SMAD2
Database Links
Gene ID: 4087 Swiss-Prot #: Q15796
OMIM #: 601366 Unigene #: Hs.12253/Hs.705764
Target Modification
Phospho
Modification Sites
H:S467 M:S467 R:S467
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Smad2 (Phospho-Ser467) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 15', using Smad2 (Phospho-Ser467) Antibody. The lane on the right is blocked with the phospho peptide.