The antiserum was produced against synthesized peptide derived from human SHP-1 around the phosphorylation site of Tyr536.
Immunogen Range
502-551
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
SHP-1 (Phospho-Tyr536) Antibody detects endogenous levels of SHP-1 only when phosphorylated at Tyr536.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PTPN6
Database Links
Gene ID: 5777 Swiss-Prot #: P29350
OMIM #: 176883 Unigene #: Hs.63489
Target Modification
Phospho
Modification Sites
H:Y536 M:Y536 R:Y538
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SHP-1 (Phospho-Tyr536) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 5', using SHP-1 (Phospho-Tyr536) Antibody. The lane on the right is blocked with the phospho peptide.