The antiserum was produced against synthesized peptide derived from human GSK3 alpha/beta around the phosphorylation site of Tyr279/216.
Immunogen Range
246-295
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
GSK3 alpha/beta (Phospho-Tyr279/216) Antibody detects endogenous levels of GSK3 alpha/beta only when phosphorylated at Tyr279/216.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
GSK3A
Database Links
Gene ID: 2931 Swiss-Prot #: P49840
OMIM #: 606784 Unigene #: Hs.466828
Target Modification
Phospho
Modification Sites
H:Y279 M:Y279 R:Y279
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GSK3 alpha/beta (Phospho-Tyr279/216) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HeLa cells, using GSK3 alpha/beta (Phospho-Tyr279/216) Antibody. The lane on the right is blocked with the phospho peptide.