Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | GSK3alpha/beta(Phospho-Tyr279/216)Antibody |
Synonyms | FactorA;GSK-3alpha;GSK3-alpha;Glycogensynthasekinase-3alpha;kinaseGSK3-alpha |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanGSK3alpha/betaaroundthephosphorylationsiteofTyr279/216. |
ImmunogenRange | 246-295 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:20000 |
Specificity | GSK3alpha/beta(Phospho-Tyr279/216)AntibodydetectsendogenouslevelsofGSK3alpha/betaonlywhenphosphorylatedatTyr279/216. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | GSK3A |
DatabaseLinks | GeneID: 2931 Swiss-Prot#: P49840 OMIM#: 606784 Unigene#: Hs.466828 |
TargetModification | Phospho |
ModificationSites | H:Y279M:Y279R:Y279 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanbreastcarcinoma,usingGSK3alpha/beta(Phospho-Tyr279/216)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromHeLacells,usingGSK3alpha/beta(Phospho-Tyr279/216)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |