Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | EGFR(Phospho-Thr678)Antibody |
Synonyms | Epidermalgrowthfactorreceptor;Proto-oncogenec-ErbB-1;Receptortyrosine-proteinkinaseerbB-1;EGFR;ERBB1 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanEGFRaroundthephosphorylationsiteofThr678. |
ImmunogenRange | 651-700 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IF:1:100~1:500ELISA:1:40000 |
Specificity | EGFR(Phospho-Thr678)AntibodydetectsendogenouslevelsofEGFRonlywhenphosphorylatedatThr678. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | EGFR |
DatabaseLinks | GeneID: 1956 Swiss-Prot#: P00533 OMIM#: 131550/211980 Unigene#: Hs.488293 |
TargetModification | Phospho |
ModificationSites | H:T678M:T680R:T679 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofHUVECcells,usingEGFR(Phospho-Thr678)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromCOS7cellstreatedwithEGF200ng/ml30",usingEGFR(Phospho-Thr678)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |