Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | AmyloidbetaA4(Phospho-Thr743/668)Antibody |
Synonyms | A4;ABPP;AD1;APP;APPI;Alzheimer"sdiseaseamyloidprotein;AmyloidbetaA4proteinprecursor;Beta-amyloidprotein40;Beta-amyloidprotein42;C31;C83;CVAP;Cerebralvascularamyloidpeptide;Gamma-CTF(50);Gamma-CTF(57);Gamma-CTF(59);P3(40);P3( |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanAmyloidbetaA4aroundthephosphorylationsiteofThr743/668. |
ImmunogenRange | 711-760 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:10000 |
Specificity | AmyloidbetaA4(Phospho-Thr743/668)AntibodydetectsendogenouslevelsofAmyloidbetaA4onlywhenphosphorylatedatThr743/668. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | APP |
DatabaseLinks | GeneID: 351 Swiss-Prot#: P05067 OMIM#: 104300/104760/605714/609065 Unigene#: Hs.434980 |
TargetModification | Phospho |
ModificationSites | H:T743M:T743R:T743 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofHeLacells,usingAmyloidbetaA4(Phospho-Thr743/668)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | Immunohistochemistryanalysisofparaffin-embeddedhumanbrain,usingAmyloidbetaA4(Phospho-Thr743/668)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image3 | WesternblotanalysisoflysatesfromHeLacells,usingAmyloidbetaA4(Phospho-Thr743/668)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |