Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | 14-3-3zeta(Phospho-Ser58)Antibody |
Synonyms | 14-3-3proteinzeta/delta;1433Z;143Z;FAS;FactoractivatingexoenzymeS;KCIP-1;MitochondrialimportstimulationfactorS1subunit;ProteinkinaseCinhibitorprotein-1;YWHAZ |
Immunogen | Theantiserumwasproducedagainstsynthesizedpeptidederivedfromhuman14-3-3zetaaroundthephosphorylationsiteofSer58. |
ImmunogenRange | 24-73 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:40000 |
Specificity | 14-3-3zeta(Phospho-Ser58)Antibodydetectsendogenouslevelsof14-3-3zetaonlywhenphosphorylatedatSer58. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | YWHAZ |
DatabaseLinks | GeneID: 7534 Swiss-Prot#: P63104 OMIM#: 601288 Unigene#: Hs.492407/Hs.594673 |
TargetModification | Phospho |
ModificationSites | H:S58M:S58R:S58 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofHeLacellstreatedwithPMA125ng/ml30",using14-3-3zeta(Phospho-Ser58)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | |
Image3 | WesternblotanalysisoflysatesfromNIH/3T3cellstreatedwithUV30",using14-3-3zeta(Phospho-Ser58)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |