Synonym:TSKgel®SizeExclusion(SW-Type)HPLCGuardColumnProperties:
RelatedCategories | Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelGelFiltrationColumnsforProteinsandotherBiopolymers,TSKgelGelFiltrationColumnstoAnalyzemAbPurityandAggregates, |
mfr.no. | TosohSuperSWHRGuard,22857 |
parameter | ≤0.5Msaltconcentration |
| 0-100%aqueoussolubleorganicsolvents |
| 1mL/minflowrate |
| 10-30°Ctemperature |
L×I.D. | 4 cm×6 mm |
matrix | silicaparticleplatform |
matrixactivegroup | diolphase |
particlesize | 4 μm |
poresize | 250 ?poresize |
operatingpH | 2.5-7.5 |
suitABIlity | suitableforL20perUSP |
| suitableforL59perUSP |
modeofchromatography | Sizeexclusion(SEC) |
Description:
Generaldescription
TheTSKgelSuperSWandUltraSWmAbcolumnsconsistofthreespecializedcolumnsdesignedfortheseparationandanalysisofmonoclonalantibodies(mAb).WhilemAbscanbeanalyzedusingmanydifferentmodesofHPLC,sizeexclusionisbesttodeterminethelevelofaggregation,dimerformation,andfragmentation,makingitthebestmethodforheterogeneitystudies.TSKgelSuperSWmAbHRcolumnsareoptimizedforhighresolutionanalysisofmAbmonomeranddimer;these30cmx7.8mmIDslica-basedstainlesssteelcolumnsofferreducedlot-to-lotvariation,longcolumnlife,reductionofunspecifiedadsorption,andsuperiorrecoveryofaggregates.TheTSKgelSuperSWmAbcolumnsutilizeauniquepore-controlledtechnology,whichproducesashallowcalibrationcurveinthemolecularweightregionofatypicalmonoclonalantibody.ThecalibrationcurvefortheTSKgelSuperSWmAbHRcolumnissimilartothatoftheindustry-leADIngTSKgelG3000SWxlcolumnandhasashallowerslopethantheTSKgelUltraSWAggregatecolumnaroundthemolecularweightrangeofgamma-globulin.Thisshallowcalibrationcurveproduceshighresolutionseparations.
Physicalform
Shippedin0.05%NaN3and0.1MNa2SO4in0.1Mphosphatebuffer,pH6.7.
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LegalInformation
TSKgelisaregisteredtrademarkofTosohCorporation