Synonym:TSKgel®HydrophobicInteractionChromatography(HIC)ColumnProperties:
RelatedCategories | Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,HydrophobicInteraction(HIC)Columns,TSKgelBioAssistPEEKHPLCColumns, |
material | PEEKhardware |
mfr.no. | Tosoh,20023 |
parameter | ≤4Msaltconcentration |
| ≤50%organicsolvent |
| 1.2mL/minflowrate |
| 10-50°Ctemperature |
| 20barmax.pressure |
application(s) | HPLC:suitable |
L×I.D. | 5 cm×7.8 mm |
matrix | polymerparticleplatform |
matrixactivegroup | phenylphase |
particlesize | 10 μm |
poresize | 1000 ? |
operatingpH | 2-12 |
modeofchromatography | Hydrophobicinteraction(HIC) |
Description:
Generaldescription
Bothhydrophobicinteractionchromatography(HIC)andreversed-phaseliquidchromatographyseparateonthebasisofproteinhydrophobicityandallowselectivebindinganddesorptionofproteins.However,HICoperatesatsignificantlylowerbindingenergyandusesaqueousmobilephases.Thesecharacteristicsprovideagentletechniquethatislesslikelytodisturbproteinconformation.Asaresult,HICgenerallyprovidesbetteractivityrecovery.
TSKgelBioAssistPhenylcolumnscontainthesameparticlesasPhenyl-5PWcolumns,butarepackedinPEEKcolumnhardwareratherthanstainlesssteel.BioAsistPhenyl(Phenyl-5PW),TSKgelEther-5PWandButyl-NPRresin-basedcolumnsprovidearangeofhydrophobicitiesforchromatographicoptimization.Ammoniumsulfateconcentrationcanbeminimizedbyusingamorehydrophobicpacking,suchasBioAssistPhenyl.WerecommendanEther-5PWcolumnforpurifyingveryhydrophobicproteins.TSKgelBioAssistPhenylandTSKgelEther-5PWpackingsarebasedonTSKgelG5000PWresin-10??μmparticleswith1000???…pores.TSKgelButyl-NPRcolumns,whicharelesshydrophobicthanEther-5PW,ispreparedfrom2.5??μmnonporousparticles,allowingforrapidanalysesandsemi-micropreparativeapplications.Allthreecolumntypescanbecleanedwith0.2??MNaOH.Scale-upcanbeperformedbyusingcorrespondingToyopearlbulkresins.
ThemosthydrophobicamongthethreeTSKgelHICcolumns,TSKgelPhenyl-5PWisanexcellentchoicetoscreenfortheselectivity,retentionandrecoveryofmostbiomolecules.Featuringa1000?poresizeinapolymethacrylatebasematerial,thePhenyl-5PWcolumnisstableineitheracidorcausticcleaningregimensandprovidesexcellentaccesstolargermoleculeswithlowdiffusioncoefficients.StainlesssteelisthestandardcolumnformatforTSKgelPhenyl-5PW.GlassandPEEKcolumnhousingsareavailableforsamplesthatmayadsorbtostainlesssteel.Thealternativecolumnformatsarealsosuitableforgeneralapplications.
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LegalInformation
BioAssistisaregisteredtrademarkofTosohCorporation
TSKgelisaregisteredtrademarkofTosohCorporation