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avanti polar lipids/12-NBD Stearate/1mg/810110P-1mg
  • avanti polar lipids/12-NBD Stearate/1mg/810110P-1mg

avanti polar lipids/12-NBD Stearate/1mg/810110P-1mg

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貨號(hào): 810110P-1mg
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    • 810110 | 12-NBD Stearate

      12-N-methyl-(7-nitrobenz-2-oxa-1,3-diazo)aminostearic acid

      12-NBD Stearate
      Info

      12-NBD Stearate

      12-N-methyl-(7-nitrobenz-2-oxa-1,3-diazo)aminostearic acid

      NBD-stearate is a fluorescent fatty acid analogue used to probe fatty acid binding protein (FABP) and sterol carrier protein-2 (SCP-2).

      Data
      Hygroscopic
      No
      Light Sensitive
      Yes
      Molecular Formula
      C25H40N4O5
      Percent Composition
      C 63.00%, H 8.46%, N 11.76%, O 16.78
      Purity
      >99%
      Stability
      1 Year
      Storage Temperature
      -20°C
      CAS Number
      117056-67-4 CAS Registry Number is a Registered Trademark of the American Chemical Society
      FormulaWeight
      476.609
      Exact Mass
      476.3
      Downloads
      • ChemDraw File
      • 3D Structure
      • Structure
      • Safety Data Sheet
      • Safety Data Sheet
      References

      Bai L, Parkin LA, Zhang H, Shum R, Previti ML, Seeliger JC. Dimethylaminophenyl Hydrazides as Inhibitors of the Lipid Transport Protein LprG in Mycobacteria. ACS Infect Dis. 2020 Mar 3. doi: 10.1021/acsinfecdis.9b00497. Epub ahead of print. PMID: 32053347.

      PubMed ID: 32053347

      Carbonetti G, Wilpshaar T, Kroonen J, Studholme K, Converso C, d"Oelsnitz S, Kaczocha M. FABP5 coordinates lipid signaling that promotes prostate cancer metastasis. Sci Rep. 2019 Dec 12;9(1):18944. doi: 10.1038/s41598-019-55418-x. PMID: 31831821; PMCID: PMC6908725.

      PubMed ID: 31831821

      Kim J, Bogdan DM, Elmes MW, Awwa M, Yan S, Che J, Lee G, Deutsch DG, Rizzo RC, Kaczocha M, Ojima I. Incarvillateine produces antinociceptive and motor suppressive effects via adenosine receptor activation. PLoS One. 2019 Jun 25;14(6):e0218619. doi: 10.1371/journal.pone.0218619. eCollection 2019.

      PubMed ID: 31237895

      Pohl J, Ring A, Stremmel W. Uptake of long-chain fatty acids in HepG2 cells involves caveolae: analysis of a novel pathway. J Lipid Res. 2002 Sep;43(9):1390-9. [PubMed]

      PubMed ID: 12235170

      Atshaves BP, Storey SM, McIntosh AL, Petrescu AD, Lyuksyutova OI, Greenberg AS, Schroeder F. Sterol carrier protein-2 expression modulates protein and lipid composition of lipid droplets. J Biol Chem. 2001 Jul 6;276(27):25324-35. [PubMed]

      PubMed ID: 11333258

      Milliano MT, Luxon BA. The peroxisomal proliferator clofibrate enhances the hepatic cytoplasmic movement of fatty acids in rats. Hepatology. 2001 Feb;33(2):413-8. [PubMed]

      PubMed ID: 11172343

      Murphy EJ. Sterol carrier protein-2 expression increases NBD-stearate uptake and cytoplasmic diffusion in L cells. Am J Physiol. 1998 Aug;275(2 Pt 1):G237-43. [PubMed]

      PubMed ID: 9688650

      Atshaves BP, Foxworth WB, Frolov A, Roths JB, Kier AB, Oetama BK, Piedrahita JA, Schroeder F. Cellular differentiation and I-FABP protein expression modulate fatty acid uptake and diffusion. Am J Physiol. 1998 Mar;274(3 Pt 1):C633-44. [PubMed]

      PubMed ID: 9530094

      Schroeder F, Myers-Payne SC, Billheimer JT, Wood WG. Probing the ligand binding sites of fatty acid and sterol carrier proteins: effects of ethanol. Biochemistry. 1995 Sep 19;34(37):11919-27. [PubMed]

      PubMed ID: 7547928

      Luxon BA. Inhibition of binding to fatty acid binding protein reduces the intracellular transport of fatty acids. Am J Physiol. 1996 Jul;271(1 Pt 1):G113-20. [PubMed]

      PubMed ID: 8760114
      Binding Info

      Data was generously provided by Dr. Huan Huang and Dr. Fred Schroeder, Texas A & M University.

      Cell Images

      Pattern of distribution in living L-cell fibroblasts

      NBD-stearate was taken up by L-cells, was not toxic at the low concentrations used, and distributed diffusely throughout the cytoplasm (at low concentrations) and both in the cytoplasm and brightly into lipid droplets (at higher concentrations).
      300nM NBD-Stearate, 15min500nM NBD-Stearate, 15min20nM Nile Red, 15min

      Photos courtesy of Dr. Huan Huang and Dr. Fred Schroeder, Texas A & M University.

      Certificates of Analysis
      • Certificate of Analysis(Lot No. 810110C-1MG-A-010and 5313CGA010)
      • Certificate of Analysis(Lot No. 810110P-1MG-A-010and 5313PGA010)
      • Certificate of Analysis(Lot No. 810110P-1MG-B-010and 5313PGB010)
      • Certificate of Analysis(Lot No. 810110P-1MG-C-010and 5313PGC010)
      • Certificate of Analysis(Lot No. 810110P-1MG-D-010and 5313PGD010)
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