ApplicationData | Detectionofhumanp38MAPkinasephosphorylatedatThr180/Tyr182,byimmunoblotting.Sample:WholecelllysatefromHEK293cellstreatedwithUVirrADIation.Primaryantibody:PhosphoDetect?Anti-p38MAPKinase(pThr180,pTyr182)RabbitpAb(Cat.No.506119)(1:1000)pre-incubatedwithpeptideimmunogen(lane1),agenericphophothreonine-containingpeptide(lane2),agenericphosphotyrosine-containgpeptide(lane3),non-phosphorylatedpeptidecorrespondingtothephosphopeptide(lane4),withoutpeptide(lane5),withphophopeptidederivedfromthecorrespondingregionofJNK1&2(lane6),andphosphopetidederivedfromthecorrespondingregionofERK1&2(lane7).Detection:chemiluminescence. |