A new preparation of the saccharolipid glycan, Kdo2-Lipid A (KLA), is a nearly homogeneous Re lipopolysaccharide (LPS) substructure with endotoxin activity equal to that of native LPS. KLA is comparable to LPS and its activity is reduced by >103 in cells from TLR-4 deficient mice. The advantage of KLA over LPS is that it is a reproducible, defined natural product, and it can be detected by ESI/MS at the low concentrations used to stimulate animal cells. The purity of KLA should also facilitate the structural analysis of its complexes with signaling receptors, such as TLR-4/MD2.
New research* shows 1μg KLAinduces
the same response as 10μg LPS.
Perform experiments at half the cost of using LPS!Avanti’s new HSA lipid delivery system, Huzzah™, improves the cellular delivery of KLA through its conjugation with human serum albumin (HSA), a physiologically relevant carrier protein. This conjugate system improves the solubility of KLA in aqueous buffer.The Huzzah™ lipid delivery system is supplied as a lyophilized conjugate which can be directly and easily reconstituted in water or a buffer of choice and is ideal for studying the effects of poorly soluble bioactive molecules.Solubility: Reconstitute lyophilized conjugate in 2 mL of water or other aqueous buffer. This produces a stock solution of 85 - 110 μg/mL KLA*. A short sonication may be necessary to ensure complete dissolution. If necessary, filter sterilize the reconstituted conjugate prior to use in cell culture applications.*Exact concentration will vary from batch to batch. Refer to label for exact product concentration upon reconstitution as determined by mass spectrometric analysis.
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