(Secondary antibodies and conjugates, affinity purified) We recommend that each investigator determine the actual optimum dilution for a given antibody or antibody conjugate for each specific assay used. Enzyme conjugates of affinity purified antibodies are supplied with lot-specific recommended dilutions. Products without specifically stated dilutions should be used according to the information below.
Conjugate | ELISA | Blotting | Flow | Tissue |
---|
|
Flourescein | nd | nd | 1:50-1:200 | 1:200-1:1000 |
Rhodamine, Texas Red | nd | nd | 1:50-1:200 | 1:200-1:1000 |
Biotin | 1:5k-1:20k | 1:3k-1:10k | nd | 1:100-1:1000 |
Peroxidase | 1:40k-1:10k | 1:2k-1:5k | nd | 1:200-1:2000 |
Alkaline phosphatase | 1:1k-1:2k | 1:500-1:1k | nd | 1:100-1:1000 |
(minimum cross-reactivity to Bv, Ch, GP, Gt, Ham, Hs, Ms, Rb, Rt & Sh serum proteins), fluorescein conjugate, lyophilized