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Millipore/17-650 | ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set/17-650/25 assays25 assays per
  • Millipore/17-650 | ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set/17-650/25 assays25 assays per

Millipore/17-650 | ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set/17-650/25 assays25 assays per

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貨號(hào): 17-650
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    • Description
      CatalogueNumber17-650
      BrandFamilyUpstate
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+Ubiquityl-HistoneH2B-ChIPValidatedAntibodyandPrimerSet
      OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+Ubiquityl-HistoneH2B,(clone56)setincludestheUbiquityl-HistoneH2B,(clone56)antibody,anegativecontrolantibody(purifiedMouseIgG),andqPCRprimerswhichamplifya213bpregionwithinthecodingregionofthehumanGAPDHgene.
      Theubiquityl-HistoneH2Bandnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofUbiquityl-HistoneH2Bassociatedchromatin.
      AlternateNames
      • H2BUb
      • HistoneH2B(ubiquityl)
      • H2Bhistonefamily,memberL
      • H2B.1A
      • histone1
      • H2bc
      • histonecluster1
      • H2bc
      ProductInformation
      FormatProteinGPurified
      Control
      • IncludesnegativecontrolmouseIgGantibodyandprimersspecificforhumanGAPDHcodingregion.
      PresentationAnti-ubiquityl-HistoneH2B,clone56(mousemonoclonalIgG).Onevialcontaining25μgofproteinGpurifiedantibodyin25μLPBScontaining0.05%sodiumazide.Storeat-20°C.

      NormalMouseIgG.Onevialcontaining25μgpurifiedmouseIgGin25μLstoragebuffercontaining0.1%sodiumazide.Storeat-20°C.

      ChIPPrimersGAPDHCodingRegion.Onevialcontaining75μLof5μMofeachprimerspecificforthecodingregionofhumanGAPDH.Storeat-20°C.
      FOR:GGCTCCCACCTTTCTCATCC
      REV:GGCCATCCACAGTCTTCTGG
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationThisChIPAb+Ubiquityl-HistoneH2B-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
      KeyApplications
      • WesternBlotting
      • Immunofluorescence
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesChromatinImmunoprecipitation:
      SonicatedchromatinpreparedfromHeLaS3cells(1X106cellequivalentsperIP)wassubjectedtochromatinimmunoprecipitationusing1μgofeitheranormalmouseIgGorAnti-Ubiquityl-HistoneH2BantibodyandtheMagnaChIPGKit(Cat.#17-611).
      Successfulimmunoprecipitationofubiquityl-HistoneH2BassociatedDNAfragmentswasverifiedbyqPCRusingGAPDHpromoter(negative)andGAPDHcoding(positive)Primers(Pleaseseefigures).DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.
      PleaserefertotheEZ-MagnaGChIP?(Cat.#17-409)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.

      WesternBlotAnalysis:
      HeLacelllysatewasresolvedbyelectrophoresis,transferredtoPVDFandprobedwithanti-Ubiquityl-histoneH2Batadilutionof1:4000(Pleaseseefigures).


      BiologicalInformation
      ImmunogenImmunogenusedwasabranchedpeptidecorrespondingtotheubiquitinationsiteonhumanhistoneH2B.
      EpitopeLys120ubiquitinationsite
      CloneClone56
      HostMouse
      SpecificityRecognizeshistoneH2Bubiquitinatedonlysine120butnotfreeubiquitinorhistoneH2Binitsunmodifiedstate.
      IsotypeIgG2aκ
      SpeciesReactivity
      • Mouse
      • Human
      • Vertebrates
      • Rat
      • GreenPlants
      SpeciesReactivityNoteHuman,mouse,rat.Nottestedinotherspecies.Theimmunogensequenceisidenticalinawiderangeofanimalandplantspecies,sobroadcross-reactivityisexpected.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneencodesamemberofthehistoneH2Bfamily,andgeneratestwotranscriptsthroughtheuseoftheconservedstem-loopterminationmotif,andthepolyAadditionmotif.[providedbyRefSeq]
      GeneSymbol
      • H2B.1
      • H2B.a/g/h/k/l
      • H2B/a
      • H2B/g
      • H2B/h
      • H2B/k
      • H2B/l
      • H2BFA
      • H2BFG
      • H2BFH
      • H2BFK
      • H2BFL
      Modifications
      • Ubiquitylation
      UniProtNumber
      UniProtSummaryFUNCTION:Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.

      Hasbroadantibacterialactivity.Maycontributetotheformationofthefunctionalantimicrobialbarrierofthecolonicepithelium,andtothebactericidalactivityofamnioticfluid.

      SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.

      SUBCELLULARLOCATION:Nucleus.

      PTM:MonoubiquitinationofLys-120bytheRNF20/40complexgivesaspecifictagforepigenetictranscriptionalactivationandisalsoprerequisiteforhistoneH3"Lys-4"and"Lys-79"methylation.ItalsofunctionscooperativelywiththeFACTdimertostimulateelongationbyRNApolymeraseII.

      PhosphorylatedonSer-15bySTK4/MST1duringapoptosis;whichfacilitatesapoptoticchromatincondensation.AlsophosphorylatedonSer-15inresponsetoDNAdoublestrandbreaks(DSBs),andincorrelationwithsomatichypermutationandimmunoglobulinclass-switchrecombination.

      SEQUENCESIMILARITY:BelongstothehistoneH2Bfamily.

      MolecularWeight~25kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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