BackgroundInformation | 4G10?Platinum Thedevelopmentoftheanti-phosphotyrosine,clone4G10?in1989wasamonumentaldiscoveryforresearchers.4G10?wasthefirstandisthebestsinglemonoclonalantibodyforthedetectiontyrosinephosphorylation.4G10?iswellknownforitssensitivityanditsABIlitytodetectmultipletyrosinephosphorylationsonnumeroussubstrates.Ithasbeenvalidatedbythousandsofscientificandmedicalresearchersinvirtuallyeveryapplicationandtyrosinetargetoverthepast2decades.Toimproveonsomethingthathundredhavetriedandnoonehassucceeded,wepooled4G10?withthenextmosthighlyregardedanti-phosphotyrosine,clonePY20tomake4G10?Platinum.PY20itselfisaverypoorsubstitutefor4G10?,butitsadditiveeffectallowforagreaterlevelofdetectiononmoresubstratesthateven4G10?alonewasnotcapableof.
4A4 TheidentificationofproteinphosphorylationasaregulatorymechanismoriginatedfromstudiesbyFischerandKrebsinthemid1950sthatlaterearnedthemthe1992Nobelprize.Itisthemajormechanismfortheregulationofdiversecellularprocessesincludingcelldivision,proteinsynthesis,transcriptionalregulationandneurotransmission.Thesteadystatephosphorylationofanygivensubstrateisgovernedbytheopposingactivitiesofkinasesandphosphatases.Itisnowbelievedthatathirdofalleukaryoticcellularproteinsarephosphorylatedandthatthemajorityofallphosphorylationeventsoccuronserineandthreonineresidues(greaterthan95%). |