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Millipore/17-10048 | ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set/17-10048/25 assays25 assays per set.
  • Millipore/17-10048 | ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set/17-10048/25 assays25 assays per set.

Millipore/17-10048 | ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set/17-10048/25 assays25 assays per set.

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貨號(hào): 17-10048
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    • Description
      CatalogueNumber17-10048
      BrandFamilyUpstate
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+HistoneH2A.Z-ChIPValidatedAntibodyandPrimerSet
      OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+HistoneH2A.ZsetincludestheHistoneH2A.Zantibody,anegativecontrolantibody(normalsheepIgG),andqPCRprimerswhichamplifya166bpregionofhumanGAPDH.TheHistoneH2A.Zandnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHistoneH2A.Z-associatedchromatin.
      AlternateNames
      • H2Ahistonefamily,memberZ
      • H2AZhistone
      BackgroundInformationH2A.Z,alsoknownasH2AFZ,isavariantofhistoneH2Avariantthatishighlyconservedfromyeasttohumans.Likeotherhistonevariants,H2A.Zisencodedbyauniquegene,resultinginaslightlydivergenttypeofH2Awithspecializedfunctions.H2A.ZisinvolvedinchromatincompactionbystABIlizingthehistoneoctamerwithinthenucleosome.Itpromotesgeneexpressionthroughthecreationofanopendomainofchromatinthatismoreeasilytranscribed.H2A.Zisnonrandomlydistributedthroughoutthegenome,targetedprimarilytopericentricheterochromatin,butexcludedfromtheinactiveXchromosomeandthenucleolus.H2A.Zisassociatedwithbotheuchromatinandfacultativeheterochromatin.DNAmethylationcaninfluencechromatinstructureandeffectgenesilencingbyexcludingH2A.Z,andlikewise,H2A.ZprotectsgenesfromDNAmethylation.Thus,H2A.Zplaysaroleinregulatingheterochromatinsilencingandisinvolvedintranscriptionalcontrol.
      ProductInformation
      FormatAffinityPurified
      Control
      • IncludesnegativecontrolsheepIgGantibodyandprimersspecificforhumanGAPDH.
      PresentationAnti-HistoneH2A.Z(SheeppolyclonalIgG).Onevialcontaining25μgofaffinitypurifiedsheeppolyclonalIgGinPBS,pH7.4containing1%BSAwith0.02%sodiumazide,beforetheadditionof30%glycerol.Storeat-20°C.

      NormalSheepIgG.Onevialcontaining25μgofproteinGpurifiedsheepIgGin0.02Mpotassiumphosphate,0.15MNaCl,pH7.2,with0.01%sodiumazide,beforetheadditionof30%glycerol.Storeat-20°C.

      ControlPrimers.Onevialcontaining75μLof5μMofeachprimerspecificforhumanGAPDH.Storeat-20°C.
      FOR:TACTAGCGGTTTTACGGGCG
      REV:TCGAACAGGAGGAGCAGAGAGCGA
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:Variabilityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationThisChIPAb+HistoneH2A.Z-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
      KeyApplications
      • ChromatinImmunoprecipitation(ChIP)
      • WesternBlotting
      ApplicationNotesChromatinImmunoprecipitation:
      SonicatedchromatinpreparedfromHeLacells(1X106cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusing1μgofeitherNormalSheepIgGor1μgofAnti-HistoneH2A.ZandtheMagnaChIP?GKit(Cat.#17-611).
      SuccessfulimmunoprecipitationofHistoneH2A.ZassociatedDNAfragmentswasverifiedbyqPCRusingControlPrimers(PartNo.22-004)forapositive,andGAPDHCodingprimersasanegativeassay(Pleaseseefigures).
      DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.
      PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.

      WesternBlotAnalysis:
      Representativelotdata
      HeLalysatewasresolvedbyelectrophoresis,transferredtoPVDFandprobedwithanti-HistoneH2A.Z(1:1000dilution).
      Proteinswerevisualizedusingarabbitanti-sheepsecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem(Pleaseseefigures).
      BiologicalInformation
      ImmunogenLinearpeptidecorrespondingtheC-terminalaminoacids113-127ofhumanHistoneH2A.Z.
      EpitopeC-terminus
      HostSheep
      SpecificityRecognizesHistoneH2A.Z,Mr~15kDa.
      SpeciesReactivity
      • Human
      SpeciesReactivityNoteHuman.Widespeciesreactivityisexpected.
      Predictedtocross-reactwithzebrafish,mouse,chicken,rat,rabbit,andXenopusbasedonsequencehomology.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneencodesamemberofthehistoneH2Afamily.Severaltranscriptvariantsencodingdifferentisoforms,havebeenidentifiedforthisgene.[providedbyRefSeq].
      GeneSymbol
      • H2A.z
      • H2A/z
      • H2AZ
      • MGC117173
      • H2AFZ
      PurificationMethodAffinityPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:VarianthistoneH2AwhichreplacesconventionalH2Ainasubsetofnucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Maybeinvolvedintheformationofconstitutiveheterochromatin.Mayberequiredforchromosomesegregationduringcelldivision.Ref.7

      SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.H2AoritsvariantH2AFZformsanheterodimerwithH2B.H2AFZinteractswithINCENPBysimilarity.

      SUBCELLULARLOCATION:Nucleus.

      PTM:MonoubiquitinationofLys-122givesaspecifictagforepigenetictranscriptionalrepression.

      AcetylatedonLys-5,Lys-8andLys-12duringinterphase.AcetylationdisappearsatmitosisBysimilarity.

      NotphosphorylatedBysimilarity.

      SIMILARITY:BelongstothehistoneH2Afamily.

      MOLECULARWEIGHT:is13413.4Dafrompositions2-128.DeterminedbyESI.Monoisotopic,notmodified.Ref.8Ref.10Ref.11

      MolecularWeight~15kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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