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Millipore/17-10045 | ChIPAb+ Acetyl-Histone H4 (Lys5) - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-100
  • Millipore/17-10045 | ChIPAb+ Acetyl-Histone H4 (Lys5) - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-100

Millipore/17-10045 | ChIPAb+ Acetyl-Histone H4 (Lys5) - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-100

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貨號(hào): 17-10045
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    • Description
      CatalogueNumber17-10045
      BrandFamilyUpstate
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+Acetyl-HistoneH4(Lys5)-ChIPValidatedAntibodyandPrimerSet,rabbitmonoclonal
      OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+Acetyl-HistoneH4(Lys5)setincludestheAcetyl-HistoneH4(Lys5)antibody,anegativecontrolsupernatant,andqPCRprimerswhichamplifya166bpregionofhumanGAPDH.TheAcetyl-HistoneH4(Lys5)andnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofAcetyl-HistoneH4(Lys5)-associatedchromatin.
      AlternateNames
      • H4K5Ac
      • HistoneH4(acetylK5)
      • H4histonefamily,memberA
      • histone1,H4a
      • histonecluster1,H4a
      BackgroundInformationHistoneH4isoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH4isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.AcetylationofhistoneH4occursatseveraldifferentlysinepositionsinthehistonetailandisperformedbyafamilyofenzymesknownasHistoneAcetylTransferases(HATs).
      ProductInformation
      FormatCultureSupernatant
      Control
      • IncludesnegativecontrolsupernatantandprimersspecificforhumanGAPDH.
      PresentationAnti-Acetyl-HistoneH4(Lys5)(rabbitmonoclonalIgG).Onevialcontaining50μLofrabbitmonoclonalIgGcellculturesupernatantin0.1%sodiumazide.Storeat-20°C.

      NegativeControlSupernatant(rabbit).Onevialcontaining50μLofculturedsupernatantin0.05%sodiumazide.Storeat-20°C.

      ControlPrimers.Onevialcontaining75μLof5μMeachprimerspecificforthehumanGAPDHpromoter.Storeat-20°C.
      FOR:TACTAGCGGTTTTACGGGCG
      REV:TCGAACAGGAGGAGCAGAGAGCGA
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationThisChIPAb+Acetyl-HistoneH4(Lys5)-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
      KeyApplications
      • WesternBlotting
      • Immunoprecipitation
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesChromatinImmunoprecipitation:
      SonicatedchromatinpreparedfromuntreatedHeLacells(1X106cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusing2μLofeitheranegativecontrolsupernatant,or2μLofAnti-Acetyl-HistoneH4(Lys5)andtheMagnaChIP?AKit(Cat.#17-610).
      Successfulimmunoprecipitationofacetyl-HistoneH4(Lys5)associatedDNAfragmentswasverifiedbyqPCRusingcontrolPrimersflankingthehumanGAPDHpromoter,orprimersamplifyingthepromoterofhumanβ-globin,whichistranscriptionallyinactiveinHeLacells(Pleaseseefigures).
      DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.

      PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.

      WesternBlotAnalysis:
      Representativelotdata.
      LysatesfromHeLacellsuntreatedorsodiumbutyratetreated(Lanes1and2respectively)wereprobedwithanti-acetyl-HistoneH4(Lys5)(1:1,000dilution).
      Proteinswerevisualizedusinggoatanti-rabbit.(Pleaseseefigures).

      WesternBlotAnalysis/PeptideInhibition:Representativelotdata.
      SodiumbutyratetreatedHeLaacidextractwasresolvedbyelectrophoresis,transferredtoPVDFmembraneandprobedwithAnti-Acetyl-HistoneH4(Lys5)(1:1000dilution).
      Todemonstratespecificity,serumwaspreincubatedwithmodifiedHistoneH4peptides:

      Lane1:Nopeptide
      Lane2:Acetyl-HistoneH4(K5)(Cat.#12-343)
      Lane3:Acetyl-HistoneH4(K8)(Cat.#12-344)
      Lane4:Acetyl-HistoneH4(K12)(Cat.#12-345)
      Lane5:Acetyl-HistoneH4(K16)(Cat.#12-346)
      Lane6:Acetyl-HistoneH4(K5,8,12,16)(Cat.#12-353)
      Lane7:HistoneH4(Cat.#12-347)

      Proteinswerevisualizedusingadonkeyanti-rabbitIgGconjugatedtoHRPandvisualizedusingachemiluminescencedetectionsystem.(Pleaseseefigures).
      BiologicalInformation
      ImmunogenPeptidecorrespondingtoHistoneH4containingthesequence[GRG-AcK-GGK]onwhichLys5isacetylated.
      EpitopeAcetylatedLys5
      HostRabbit
      SpecificityRecognizesHistoneH4acetylatedonLys5.
      IsotypeIgG
      SpeciesReactivity
      • Human
      SpeciesReactivityNoteHuman.Widerangeofcross-reactivityexpectedbasedonsequencehomology.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Thisstructureconsistsofapproximately146bpofDNAwrappedaroundanucleosome,anoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtails;instead,theycontainapalindromicterminationelement.Thisgeneisfoundinahistoneclusteronchromosome1.Thisgeneisoneoffourhistonegenesintheclusterthatareduplicated;thisrecordrepresentsthetelomericcopy.[providedbyRefSeq].
      GeneSymbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P62805#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
      SIZE:103aminoacids;11367Da
      SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
      SUBCELLULARLOCATION:Nucleus.
      PTM:SymmetricdimethylationonArg-4bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage(Bysimilarity).&UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirrADIation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins.
      SIMILARITY:SwissProt:P62805##BelongstothehistoneH4family.
      MolecularWeight~11kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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