Source | producedinE.coli |
SpecificActivity | ForSpecificActivitydata,refertotheCertificateofAnalysisforindividuallotsofthisenzyme. |
EntrezGeneNumber | |
EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.Thisgeneencodesareplication-independentmemberofthehistoneH2Afamilythatisdistinctfromothermembersofthefamily.StudiesinmicehaveshownthatthisparticularhistoneisrequiredforembryonicdevelopmentandindicatethatlackoffunctionalhistoneH2Aleadstoembryoniclethality. |
GeneSymbol | - H2AFZ
- H2A/z
- H2A.Z
- MGC117173
- H2AZ
- H2A.z
|
PurificationMethod | Ion-exchangechromatography |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P0C0S5#VarianthistoneH2AwhichreplacesconventionalH2Ainasubsetofnucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Maybeinvolvedintheformationofconstitutiveheterochromatin.Mayberequiredforchromosomesegregationduringcelldivision. SIZE:128aminoacids;13553Da SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.H2AoritsvariantH2AFZformsanheterodimerwithH2B.H2AFZinteractswithINCENP(Bysimilarity). SUBCELLULARLOCATION:Nucleus. PTM:MonoubiquitinationofLys-122givesaspecifictagforepigenetictranscriptionalrepression.&AcetylatedonLys-5,Lys-8andLys-12duringinterphase.Acetylationdisappearsatmitosis(Bysimilarity).&Notphosphorylated(Bysimilarity). SIMILARITY:SwissProt:P0C0S5##BelongstothehistoneH2Afamily. |
MolecularWeight | 16kDa |