GoldBio/CSF2 (GM-CSF), Murine/250 μg/1320-03-250

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

Recombinant Murine Colony Stimulating Factor 2 (granulocyte-macrophage)CSF2GM-CSF, GMCSFMolgramostinMGI-1GM (Macrophage granulocyte inducer-1GM)

Colony Stimulating Factor 2 (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts, in response to cytokine or immune and inflammatory stimuli. On mature hematopoietic cells, CSF2 is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. CSF2 promotes a Th1 biased immune response, angiogenesis, allergic inflammation and the development of autoimmunity. It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and CSF2 transfected tumor cells are utilized as cancer vaccines.Mature mouse CSF2 shares 49% - 54% amino acid sequence identity with canine, feline, human, and porcine CSF2 and 69% with rat CSF2.The activity of CSF2 is species specific between human and mouse. Mouse CSF2 is only weakly active on rat cells, although rat CSF2 is fully active on mouse cells.

Formulation: Sterile filtered whitelyophilized powder. Purified and tested for use in cell culture.

Sequence: APTRSPITVTRPWKHVEAIK EALNLLDDMP VTLNEEVEVV SNEFSFKKLT CVQTRLKIFE QGLRGNFTKL KGALNMTASYYQTYCPPTPE TDCETQVTTY ADFIDSLKTF LTDIPFECKK PVQK

MW: ~14.2 kDa (124 amino acid)

Purity: >95% by SDS-PAGEand HPLC analyses

Biological Activity: Fully biologicallyactive when compared to standard. The ED₅₀ as determined by a cellproliferation assay using murine FDC-P1 cells is less than 0.05 ng/ml,corresponding to a specific activity of >2.0 × 10⁷ IU/mg.

Accession Number: P01587

MGI ID: 1339752

Source: Escherichia coli

Storage/Handling: This lyophilizedpreparation is stable at 2-8°C, but should be kept at -20°C for long termstorage. The reconstituted sample can be apportioned into working aliquots andstored at -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.

Reconstitution: The sample shouldbe briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitutein a siliconized tube using PBS that contains a 0.1% BSA to a concentration of0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C.Stock solutions should be aliquoted and stored at -80°C. Further dilutionsshould be made in appropriate buffered solutions containing BSA or serum.

Additional resources for this product may be found at:

OMIM

NOT FOR HUMAN USE!