CytocaptureH20-10productswithmicroscalecavitiesareusedforhighresolutionandlifecellimagingapplicationsofseparatedandarrestednonadherentcellssmallerthan15μmindiameterorcellnuclei.Theymaybeusedforapplicationslike:
- Sensitivefluorescenceanalysis(FRET,FRAP,FLIM)
- Highandsuperresolutionmicroscopy
- Translocationstudies
- Cellsignalingstudies
- Timelapsevideomicroscopyofnon-adherentcells
- Individualcellcharacterizationandpicking
TheCytoCaptureH20-10productshavebeendesignedforcell-basedassaysandhighresolutionlifecellimagingofsinglenonadherentcells.Auniformarrayofhexagonalpolymercavitieslaysontopofthecoverglassbottomsofthesespecializedcellcultureandimagingconsumables.Seededcellssedimentspontaneouslyinsidethemicrocavities.Thediameterofthecavitiesallowsonlysmallsinglecellstoenter.Withinthesecavitiesthecellsareprotectedfromgeneralfluidconvectioninthelabware.Thereforemediumexchangeandstainingprocedurescanbeperformedandindividualcellscanbetrackedoverdayswithoutchangeoftheirposition.Thetechnologyenableshighresolutionandintracellularmicroscopyofnon-adherentcellsinlifecellexperimentsandevenafterfixationandstaining.