ThekitconsistsofAnti-Biotin
MACSiBead?ParticlesandbiotinylatedantibodiesagainstmouseCD3εandCD28.Anti-BiotinMACSiBeadParticlesloadedwithbiotinylatedantibodiesareusedtomimicantigen-presentingcellsandactivaterestingTcellsinsingle-cells
USPensionsfromspleenorlymphnodesaswellaspurifiedTcells.Tcellexpansionisachievedbyculturingandreactivationatday6–8ofculture.