PBST (phosphate buffered solution) is a phosphate buffer containing Tween-20, which can provide a relatively stable ionic environment and pH buffering capacity. It is a buffered salt solution often used in biology and is mainly used for immunohistochemistry and in situ In experiments such as hybridization and enzyme-linked immunoassay, wash the western blot membrane and so on.?The product is a colorless liquid with a pH of 7.2-7.4 (25°C). The main ingredients are potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, potassium chloride and Tween-20.
This product is 10× stock solution and cannot be used directly. It needs to be diluted to 1× working solution.?Storage at 4°C can extend the shelf life of the product. Low temperature storage is not recommended for long-term storage.
Precautions
Low temperature storage is easier to produce precipitation.?If there is precipitation, please shake or place it in a 37℃ water bath until it is completely dissolved before use.
For your safety and health, please wear lab coats and disposable gloves.
A 10X Concentrated solution of Phosphate Buffered Saline with Tween? 20. The 10X Concentration is 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween? 20, pH 7.4.
PBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The Phosphate Buffered Saline with Tween? 20 is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter plate wells without disrupting the antigen/antibody binding reaction.
By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.
Features
1X Concentration is 8mM Na2HPO4, 150mM NaCl, 2mM KH2PO4, 3mM KCl, 0.05% Tween? 20, pH 7.4Applications
Wash buffer for Western Blotting and ELISA
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