Immunogen | KLH-conjugatedlinearpeptidecorrespondingtothematureproteinofhumanSIRT2. |
Epitope | Matureprotein |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Rabbit |
Specificity | ThisantibodyrecognizesthematureproteinofSIRT2. |
SpeciesReactivity | |
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Thisgeneencodesamemberofthesirtuinfamilyofproteins,homologstotheyeastSir2protein.Membersofthesirtuinfamilyarecharacterizedbyasirtuincoredomainandgroupedintofourclasses.Thefunctionsofhumansirtuinshavenotyetbeendetermined;however,yeastsirtuinproteinsareknowntoregulateepigeneticgenesilencingandsuppressrecombinationofrDNA.Studiessuggestthatthehumansirtuinsmayfunctionasintracellularregulatoryproteinswithmono-ADP-ribosyltransferaseactivity.TheproteinencodedbythisgeneisincludedinclassIofthesirtuinfamily.Twotranscriptvariantsresultfromalternativesplicingofthisgene. |
GeneSymbol | |
PurificationMethod | AffinityPurfied |
UniProtNumber | |
UniProtSummary | FUNCTION:NAD-dependentdeacetylase,whichdeacetylatesthe"Lys-40"ofalpha-tubulin.Involvedinthecontrolofmitoticexitinthecellcycle,probablyviaitsroleintheregulationofCytoskeleton.DespitesomeABIlitytodeacetylatehistonesinvitro,itisunlikelyinvivo.
CATALYTICACTIVITY:NAD++anacetylprotein=nicotinamide+O-acetyl-ADP-ribose+aprotein.
COFACTOR:Binds1zincionpersubunit.
ENZYMEREGULATION:InhibitedbySirtinol,A3andM15smallmolecules.Inhibitedbynicotinamide.
SUBUNITSTRUCTURE:InteractswithHDAC6,suggestingthattheseproteinsbelongtoalargecomplexthatdeacetylatethecytoskeleton.
SUBCELLULARLOCATION:Cytoplasm?cytoskeleton.Note:Colocalizeswithmicrotubules.
TISSUESPECIFICITY:Widelyexpressed.Highlyexpressedinheart,brainandskeletalmuscle,whileitisweaklyexpressedinplacentaandlung.Down-regulatedinmanygliomassuggestingthatitmayactasatumorsuppressorgeneinhumangliomaspossiblythroughtheregulationofmicrotubulenetwork.
DEVELOPMENTALSTAGE:Peaksduringmitosis.Aftermitosis,itisprobablydegradedbythe26Sproteasome.
PTM:PhosphorylatedattheG2/Mtransitionofthecellcycle.
SEQUENCESIMILARITIES:Belongstothesirtuinfamily.
Contains1deacetylasesirtuin-typedomain.
SEQUENCECAUTION:ThesequenceAAF67015.1differsfromthatshown.Reason:Frameshiftatseveralpositions. |
MolecularWeight | Observedmolecularweightis~39kDa.Multipleisoformsaround40kDamaybeobserved. |