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Millipore/06-734 | Anti-β-Catenin Antibody/06-734/200 µg
  • Millipore/06-734 | Anti-β-Catenin Antibody/06-734/200 µg

Millipore/06-734 | Anti-β-Catenin Antibody/06-734/200 µg

價(jià)格: ￥4440.00 市場(chǎng)價(jià): 7400.00
貨號(hào): 06-734
品牌: Millipore
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    • Description
      CatalogueNumber06-734
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-β-CateninAntibody
      AlternateNames
      • catenin(cadherin-associatedprotein)
      • beta1(88kD)
      • catenin(cadherin-associatedprotein),beta1(88kDa)
      • catenin(cadherin-associatedprotein),beta1,88kDa
      BackgroundInformationβ-cateninplaysacentralroleincelladhesion,bridgingbetweencadherinsandactinCytoskeleton.Theseactivitiesareregulatedthroughitsdifferentialinteractionwithseveralmolecularpartners,includingcell-celladherensjunction(AJ)molecules,componentsofitsdegradationcomplex,andtranscriptionfactors.
      β-Cateniniscomposedofthreedomains:acentralarmADIllorepeatdomainandtwoN-andC-terminaltails.β-catenincanbephosphorylatedbyserine-threoninekinase,suchasGSK-3.Thephosphorylatedβ-cateninaffectstheproteinassociationofβ-catenintoE-cadherinandα-cateininthatoccursinthecell.Thisprocesscanbereversedbyde-phosphorylationofβ-catenin.
      ProductInformation
      FormatPurified
      Control
      • A431celllysate
        IncludedPositiveAntigenControl:Catalog#12-301,NonstimulatedA431celllysate.Add2.5μLof2-mercaptoethanol/100μLoflysateandboilfor5minutestoreducethepreparation.Load20μgofreducedlysateperlaneforminigels.
      PresentationPurifiedrabbitpolyclonalIgGinbuffercontaining0.1MTrisGlycine,0.15MNaCl,0.05%SodiumAzide,pH7.4.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
      HandlingRecommendations:Priortoremovingthecap,centrifugethevialandgentlymixthesolution.
      Applications
      ApplicationUseAnti-β-CateninAntibody(RabbitPolyclonalAntibody)validatedinICC,IP,WBtodetectβ-Cateninalsoknownascatenin(cadherin-associatedprotein)beta1(88kD).
      KeyApplications
      • Immunocytochemistry
      • Immunoprecipitation
      • WesternBlotting
      ApplicationNotesImmunoprecipitation:
      5μgofapreviouslotimmunoprecipitatedβ-Cateninfrom500μgofA431membranefractionlysate.

      Immunocytochemistry:
      1-5μg/mLofapreviouslotshowedpositiveimmunostainingforβ-cateninatcell-celljunctionsofA431cellsfixedwith4%paraformaldehydeandpermeABIlizedwith0.25%Triton?X-100.
      BIOLOGicalInformation
      ImmunogenKLH-conjugated,syntheticpeptide(CGG-SYLDSGIHSGATTTAPSLSGK)correspondingtotheconsensusGSK3phosphorylationsiteofhumanβ-Catenin(aminoacids29-49).
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      SpecificityRecognizesβ-Catenin,Mr92kDa.
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Rat
      • Mouse
      • Rabbit
      • Bovine
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryBeta-cateninisanadherensjunctionprotein.Adherensjunctions(AJs;alsocalledthezonulaadherens)arecriticalfortheestablishmentandmaintenanceofepitheliallayers,suchasthoseliningorgansurfaces.AJsmediateadhesionbetweencells,communicateasignalthatneighboringcellsarepresent,andanchortheactincytoskeleton.Inservingtheseroles,AJsregulatenormalcellgrowthandbehavior.Atseveralstagesofembryogenesis,woundhealing,andtumorcellmetastasis,cellsformandleaveepithelia.Thisprocess,whichinvolvesthedisruptionandreestablishmentofepithelialcell-cellcontacts,mayberegulatedbythedisassemblyandassemblyofAJs.AJsmayalsofunctioninthetransmissionofthe"contactinhibition"signal,whichinstructscellstostopdividingonceanepithelialsheetiscomplete.[suppliedbyOMIM]
      GeneSymbol
      • CTNNB1
      • CTNNB
      • Beta-catenin
      • PRO2286
      • FLJ37923
      • FLJ25606
      • DKFZp686D02253
      PurificationMethodProteinAchromatography
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P35222#InvolvedintheregulationofcelladhesionandinsignaltransductionthroughtheWntpathway.
      SIZE:781aminoacids;85497Da
      SUBUNIT:Twoseparatepoolsarefoundinthecytoplasm:oneisPSEN1/cadherin/catenincomplexwhichanchorstotheactincytoskeleton.TheotherpoolispartofalargecomplexcontainingAXIN1,AXIN2,APC,CSNK1A1andGSK3BthatpromotesphosphorylationonN-terminalSerandThrresiduesandubiquitinationofCTNNB1viaBTRCanditssubsequentdegradationbytheproteasome.Wnt-dependentactivationofDVLantagonizestheactionofGSK3B.WhenGSK3Bactivityisinhibitedthecomplexdissociates,CTNNB1isdephosphorylatedandisnolongertargetedfordestruction.Thestabilizedproteintranslocatestothenucleus,whereitbindsTCF/LEF-1familymembers,TBP,BCL9andpossIBLyalsoRUVBL1andDUPLIN.BindsCTNNBIPandEP300.CTNNB1formsaternarycomplexwithLEF1andEP300thatisdisruptedbyCTNNBIP1binding(Bysimilarity).InteractswiththePDZdomainofTAX1BP3;thisinteractioninhibitsthetranscriptionalactivityofCTNNB1(Bysimilarity).InteractswithAJAP1,BAIAP1,CARM1,CTNNA3,CXADRandPCDH11Y.BindsSLC9A3R1.InteractswithGLIS2.InteractswithSLC30A9(Bysimilarity).
      SUBCELLULARLOCATION:Cytoplasm.Nucleus.Note=Cytoplasmicwhenitisunstabilized(highlevelofphosphorylation)orboundtoCDH1.Translocatestothenucleuswhenitisstabilized(lowlevelofphosphorylation).InteractionwithGLIS2promotesnucleartranslocation.
      TISSUESPECIFICITY:Expressedinseveralhairfolliclecelltypes:basalandperipheralmatrixcells,andcellsoftheouterandinnerrootsheats.Expressedincolon.
      PTM:PhosphorylationbyGSK3BrequirespriorphosphorylationofSer-45byanotherkinase.PhosphorylationproceedsthenfromThr-41toSer-37andSer-33.&EGFstimulatestyrosinephosphorylation.PhosphorylationonTyr-654decreasesCDH1bindingandenhancesTBPbinding.&UbiquitinatedbyaE3ubiquitinligasecomplexcontainingUBE2D1,SIAH1,CACYBP/SIP,SKP1A,APCandTBL1X(Probable).Itsubiquitinationleadstoitssubsequentproteasomaldegradation.
      DISEASE:"SwissProt:P35222#ActivatingmutationsinCTNNB1haveoncogenicactivityresultingintumordevelopment.Somaticmutationsarefoundinvarioustumortypes,includingcoloncancers,ovarianandprostatecarcinomas,hepatoblastoma(HB),hepatocellularcarcinoma(HCC).HBsaremalignantembryonaltumorsmainlyaffectingyoungchildreninthefirstthreeyearsoflife.&DefectsinCTNNB1areacauseofpilomatrixoma(PTR)[MIM:132600];acommonbenignskintumor.&DefectsinCTNNB1areacauseofmedulloblastoma(MDB)[MIM:155255].MDBisamalignant,invasiveembryonaltumorofthecerebellumwithapreferentialmanifestationinchildren.&AchromosomalrearrangementinvolvingCTNNB1maybeacauseofsalivaryglandpleiomorphicadenomas(PA)[181030].Pleiomorphicadenomasarethemostcommonbenignepithelialtumorsofthesalivarygland.Translocationt(3;8)(p21;q12)withPLAG1."
      SIMILARITY:SwissProt:P35222##Belongstothebeta-cateninfamily.&Contains12ARMrepeats.
      MolecularWeight92kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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