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Millipore/06-1352 | Anti-acetyl Histone H4 (Lys12) Antibody/06-1352/100 µL
  • Millipore/06-1352 | Anti-acetyl Histone H4 (Lys12) Antibody/06-1352/100 µL

Millipore/06-1352 | Anti-acetyl Histone H4 (Lys12) Antibody/06-1352/100 µL

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貨號: 06-1352
品牌: Millipore
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    • Description
      CatalogueNumber06-1352
      DescriptionAnti-acetylHistoneH4(Lys12)Antibody
      AlternateNames
      • H4K12Ac
      • HistoneH4(acetylK12)
      • H4histonefamily,memberA
      • histone1,H4a
      • histonecluster1,H4a
      BackgroundInformationHistoneH4isoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH4isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.AcetylationofhistoneH4occursatseveraldifferentlysinepositionsinthehistonetailandisperformedbyafamilyofenzymesknownasHistoneAcetylTransferases(HATs).
      ProductInformation
      FormatSerum
      Control
      • SodiumbutyratetreatedanduntreatedHeLacelllysate
      PresentationUnpurifiedrabbitpolyclonalinserumwith0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.
      HandlingRecommendations:Uponreceiptandpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationUseAnti-acetylHistoneH4(Lys12)Antibody(RabbitPolyclonalAntibody)validatedinWB,ICCtodetectacetylHistoneH4(Lys12)alsoknownasH4K12Ac,HistoneH4(acetylK12).
      KeyApplications
      • WesternBlotting
      • Immunocytochemistry
      ApplicationNotesImmunocytochemistryAnalysis:1:500dilutionfromarepresentativelotdetectedHistoneH4inHeLaandA431cells.
      BIOLOGicalInformation
      ImmunogenLinearpeptidecorrespondingtoArABIdopsisthalianaHistoneH4acetylatedatandaroundacetylatedLys12.
      EpitopeLys12
      HostRabbit
      SpecificityThisantibodyrecognizesHistoneH4acetylatedinandaroundLys12.
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      • Drosophila
      SpeciesReactivityNoteDemonstratedtoreactwithhuman,mouse,andrat.PredictedtoreactwithArabidopsisthaliana(higherplant)andDrosophilabasedon100%sequencehomology.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Acetylation
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.

      SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.

      SUBCELLULARLOCATION:NucleusBysimilarity.

      SEQUENCESIMILARITIES:BelongstothehistoneH4family.
      MolecularWeight~14kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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