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Millipore/05-915 | Anti-N-Cadherin Antibody, clone 13A9/05-915/100 µL
  • Millipore/05-915 | Anti-N-Cadherin Antibody, clone 13A9/05-915/100 µL

Millipore/05-915 | Anti-N-Cadherin Antibody, clone 13A9/05-915/100 µL

價(jià)格: ￥4068.00 市場價(jià): 6780.00
貨號: 05-915
品牌: Millipore
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    • Description
      CatalogueNumber05-915
      Replaces04-1126
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-N-CadherinAntibody,clone13A9
      AlternateNames
      • Cadherin-2
      • CD325
      • CDw325
      • N-cadherin
      • Neuralcadherin
      BackgroundInformationCadherin-2(UniProtP19022;alsoknownasCD325,CDw325,N-cadherin,Neuralcadherin)isencodedbytheCDH2(alsoknownasCDHN,NCAD)gene(GeneID1000)inhuman.Cadherinsconstituteafamilyofcalcium-dependentcell-celladhesionproteinsthatplayimportantrolesintheembryonicdevelopmentandmaintenanceofnormaltissuearchitecture.Cadherinsarecomposedofanextracellulardomain(a.a.160-724ofhumanN-cadherin)withfivehomologousrepeatsthatmediatesadhesion,asinglepasstransmembranedomain(a.a.725-745ofhumanN-cadherin),andaconservedcytoplasmicdomain(a.a.746-906ofhumanN-cadherin)thatinteractswithcateninstolinkcadherinstotheactinCytoskeleton.Inaddition,aknownSrcsubstratep120ctnalsomodulatethestrengthofcadherin-dependentadhesionbyinteractingwithcadherinsattheirintracellularjuxtamembranedomain.Cadherinsaresynthesizedasprecursorproteinsthatmustbeproteolyticallycleavedtogeneratefunctional,matureproteins.NewlysynthesizedproN-cadherin(a.a.1-906)isphosphorylatedandproteolyticallyprocessedpriortotransporttotheplasmamembrane.Inaddition,Plakoglobin(gamma-catenin)andbeta-cateninassociateonlywithphosphorylatedproN-cadherin,whereasp120ctncanassociatewithbothphosphorylatedandnon-phosphorylatedproN-cadherin.TheN-terminalsignalandpropeptide(a.a.1-25and26-159ofofhumanN-cadherin)regionisproteolyticallyremovedandacoreN-cadherin-catenincomplexisassembledintheendoplasmicreticulumorGolgicompartmentpriortolocalizationattheplasmamembranewherelinkagetotheactincytoskeletoncanbeestablished.
      ProductInformation
      FormatCultureSupernatant
      Control
      • HeLacelllysate
      PresentationMousemonoclonalimmunoglobulinhybridomaculturesupernatantcontaining0.05%sodiumazidebeforetheadditionofglycerolto30%.
      StorageandShippingInformation
      StorageConditionsMaintainfor2yearsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationDetectN-cadherinusingthisAnti-N-CadherinAntibody,clone13A9validatedforuseinImmunocytochemistry,Immunohistochemistry,Immunoprecipitation,andWesternBlotting.
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • WesternBlotting
      ApplicationNotesImmunohistochemistryAnalysis:Arepresentativelotimmunostainedtheextracellularmatrixofthestableplaquesandinthefibrouscapregionrichinvascularsmoothmusclecells(VSMCs)usingpatients-derivedparaf?n-embeddedinternalcarotidarterytissuesections(Musumeci,G.,etal.(2014).Histol.Histopathol.29(6):707-719).
      ImmunohistochemistryAnalysis:ArepresentativelotdetectedstrongN-cadherinimmunoreactivityinparaf?n-embeddedrectalcancer(RC)tissueswithpositiveregionallymphnodemetastasis(RLNM)status,whileonlyweakN-cadherinimmunoreactivitywasdetectedinRCwithnegativeRLNM,andnoN-cadherinstainingwasseeninnormalcolorectalepithelium(Fan,X.J.,etal.(2012).Br.J.Cancer.106(11):1735-1741).
      ImmunohistochemistryAnalysis:ArepresentativelotdetectedN-cadherinimmunoreactivityinformalin-?xed,paraf?n-embeddedhepatocellularcarcinoma(HCC)tissuesections.Asigni?cantinversecorrelationwasfoundbetweenRUNX3andN-cadherinexpressionlevels(Tanaka,S.,etal.(2012).Int.J.Cancer.131(11):2537-2546).
      WesternBlottingAnalysis:ArepresentativelotdetectedanupregulatedN-cadherinexpressioninCCL185carcinomacellsfollowingtransientEpstein-Barrvirus(EBV)infection.TheEMT-likephenotyperemainedevenaftervirallossbycultureselectionpressurewithdrawal(Queen,K.J.,etal.(2013).Int.J.Cancer.132(9):2076-2086).
      WesternBlottingAnalysis:ArepresentativelotdetectedN-cadherininHep3B,Huh7,HLFandSK-Hep1humanhepatocellularcarcinoma(HCC)celllysates(Tanaka,S.,etal.(2012).Int.J.Cancer.131(11):2537-2546).
      WesternBlottingAnalysis:Arepresentativelotdetectedboththeunprocessed(pro-)andprocessed(mature)formsofN-cadherininHeLacelllysate(Wahl,J.K.3rd.,etal.(2003).J.Biol.Chem.278(19):17269-17276).
      WesternBlottingAnalysis:ArepresentativelotdetectedN-cadherininWI-38humanfibroblastlysate,butnotinJArhumanplacentalchoriocarcinomacelllysate(Knudsen,K.A.,etal.(1995).J.CellBiol.130(1):67-77).
      ImmunocytochemistryAnalysis:ArepresentativelotdetectedN-cadherinimmunoreactivitylocalizedprimarilyatthecell-cellbordersbyfluorescentimmunocytochemistrystainingof1%paraformaldehyde-fixed,methanol-permeABIlizedHeLacells(Wahl,J.K.3rd.,etal.(2003).J.Biol.Chem.278(19):17269-17276).
      ImmunocytochemistryAnalysis:ArepresentativelotdetectedN-cadherinimmunoreactivitycolocalizedwiththoseofalpha-andbeta-cateninbydualfluorescentimmunocytochemistrystainingoffixedWI-38humanfibroblasts(Knudsen,K.A.,etal.(1995).J.CellBiol.130(1):67-77).
      ImmunoprecipitationAnalysis:Representativelotsco-immunoprecipitatedalpha-catenin,beta-catenin,andplakoglobinwithN-cadherinfromWI-38humanfibroblastandHeLacelllysates(Wahl,J.K.3rd.,etal.(2003).J.Biol.Chem.278(19):17269-17276;Knudsen,K.A.,etal.(1995).J.CellBiol.130(1):67-77).
      BIOLOGicalInformation
      ImmunogenBacteriallyexpressedhumanN-cadherincytoplasmicdomainMBPfusionprotein(Knudsen,K.A.,etal.(1995).J.CellBiol.130(1):67-77).
      EpitopeCytoplasmicdomain.
      Clone13A9
      HostMouse
      SpecificityClone13A9recognizesN-cadherin,butnotP-,E-,orM-cadherin(Knudsen,K.A.,etal.(1995).J.CellBiol.130(1):67-77).
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • CDH2
      • CDHN
      • NCAD
      PurificationMethodUnpurified
      UniProtNumber
      MolecularWeight~140kDaobserved.Targetbandsizeappearslargerthanthecalculatedmolecularweightsof82.03kDa(mature)and99.81/97.04kDa(isoform1/2pro-form)duetoposttranslationalglycosylationandphosphorylation.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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