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Millipore/05-802R | Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal/05-802R/100 µL
  • Millipore/05-802R | Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal/05-802R/100 µL

Millipore/05-802R | Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal/05-802R/100 µL

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貨號(hào): 05-802R
品牌: Millipore
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    • Description
      CatalogueNumber05-802R
      Replaces05-802
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-phospho-Akt(Thr308)Antibody,clone50-1C-25,rabbitmonoclonal
      AlternateNames
      • ProteinkinaseB
      • RAC-alphaserine/threonine-proteinkinase
      • murinethymomaviral(v-akt)oncogenehomolog-1
      • racproteinkinasealpha
      • v-aktmurinethymomaviraloncogenehomolog1
      BackgroundInformationAkt/PKBisaSer/ThrkinaseandamajorknowneffecterofthePI3Kinasepathway.ItisinvolvedinmultiplesignalingpathwaysthatrelatetomanyBIOLOGicalprocessesincludingmetabolism,apoptosis,cellcyclecontrol,angiogenesis,differentiation,andcellgrowthandproliferation.Inmammals,threeisoformsofAkt(Akt1/PKCα,Akt2/PKBβ,andAkt3/PKBγ)exists.Theyexhibitahighdegreeofhomology,butdifferslightlyinthelocalizationoftheirregulatoryphosphorylationsites.Akt1isthepredominantisoformthatisinmosttissuesandisthoughttohaveadominantroleingrowth,survival,embryonicdevelopment,andADIpocytedifferentiation.Akt2iscorrelatedwiththeregulationofglucosehomeostasisandisthepredominantisoformexpressedininsulin-responsivetissues.Akt3isabundantinbraintissue.EachAktisoformiscomposedofthreefunctionallydistinctregions:anN-terminalPleckstrinHomology(PH)domainthatprovidesalipid-bindingmodule,acentralcatalyticdomaincontainingThr308,andaC-terminalhydrophobicmotifcontainingSer473.TheactivationofAKTisdependentonadualregulatorymechanismthatrequiresbothitstranslocationtotheplasmamembraneanddualphosphorylationonThr308andSer473byPDK1andtheTORC2complex,respectively.
      ProductInformation
      FormatPurified
      PresentationProteinApurifiedrecombinantrabbitmonoclonalsupernatantin0.1MTris-Glycine(pH7.4)150mMNaCl,and0.05%NaN3with40%glycerol.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.
      HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationDetectphospho-Akt(Thr308)usingthisAnti-phospho-Akt(Thr308)Antibody,clone50-1C-25validatedforuseinWB,IF,IC,ELISA.
      KeyApplications
      • WesternBlotting
      • Immunofluorescence
      • Immunocytochemistry
      • ELISA
      ApplicationNotesWesternBlotAnalysis:
      ThislotdetectedAktwhenphosphorylatedonThr308at1:1,000dilutioninPDGF-treatedNIH/3T3lysatesviaSDS-PAGEandtransferredtoPVDF(Immobilon-P).
      BiologicalInformation
      ImmunogenKLH-conjugatedsyntheticpeptidecontainingtheThr308sequenceandsurroundingaminoacidsofhuman,mouse,rat,bovineandchickenAkt1.
      CloneNL50
      HostRabbit
      SpecificityDetectsAktonlywhenphosphorylatedonThr308.
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Mouse
      • Rabbit
      • Bovine
      • Chicken
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheserine-threonineproteinkinaseencodedbytheAKT1geneiscatalyticallyinactiveinserum-starvedprimaryandimmortalizedfibroblasts.AKT1andtherelatedAKT2areactivatedbyplatelet-derivedgrowthfactor.Theactivationisrapidandspecific,anditisabrogatedbymutationsinthepleckstrinhomologydomainofAKT1.Itwasshownthattheactivationoccursthroughphosphatidylinositol3-kinase.InthedevelopingnervoussystemAKTisacriticalmediatorofgrowthfactor-inducedneuronalsurvival.Survivalfactorscansuppressapoptosisinatranscription-independentmannerbyactivatingtheserine/threoninekinaseAKT1,whichthenphosphorylatesandinactivatescomponentsoftheapoptoticmachinery.Multiplealternativelysplicedtranscriptvariantshavebeenfoundforthisgene.[providedbyRefSeq]
      GeneSymbol
      • AKT
      • C-AKT
      • EC2.7.11.1
      • MGC99656
      • PKB
      • PKB-ALPHA
      • PRKBA
      • RAC
      • RAC-ALPHA
      • RAC-PK-alpha
      Modifications
      • Phosphorylation
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P31749#Generalproteinkinasecapableofphosphorylatingseveralknownproteins.PhosphorylatesTBC1D4.Signalsdownstreamofphosphatidylinositol3-kinase(PI(3)K)tomediatetheeffectsofvariousgrowthfactorssuchasplatelet-derivedgrowthfactor(PDGF),epidermalgrowthfactor(EGF),insulinandinsulin-likegrowthfactorI(IGF-I).Playsaroleinglucosetransportbymediatinginsulin-inducedtranslocationoftheGLUT4glucosetransportertothecellsurface.MediatestheantiapoptoticeffectsofIGF-I.Mediatesinsulin-stimulatedproteinsynthesis,partlybyplayingaroleinbothinsulin-inducedphosphorylationof4E-BP1andininsulin-inducedactivationofp70S6kinase.Promotesglycogensynthesisbymediatingtheinsulin-inducedactivationofglycogensynthase.
      SIZE:480aminoacids;55686Da
      SUBUNIT:InteractswithCENTG1isoform2(PIKE-A)inthepresenceofguaninenucleotides.TheC-terminusinteractswithCCDC88A/GRDN.InteractswithAKTIP.
      SUBCELLULARLOCATION:Cytoplasm.Nucleus.Note=Nucleusafteractivationbyintegrin-linkedproteinkinase1(ILK1).
      TISSUESPECIFICITY:Inallhumancelltypessofaranalyzed.
      DOMAIN:SwissProt:P31749BindingofthePHdomaintothephosphatidylinositol3-kinasealpha(PI(3)K)resultsinitstargetingtotheplasmamembrane.
      PTM:PhosphorylationonThr-308,Ser-473andTyr-474isrequiredforfullactivity.Ser-473phosphorylationbytheRictor-mTorcomplexfavorsThr-308phosphorylationbyPDPK1.Ser-473phosphorylationisenhancedbyinteractionwithCENTG1isoform2(PIKE-A).
      SIMILARITY:Belongstotheproteinkinasesuperfamily.AGCSer/Thrproteinkinasefamily.RACsubfamily.&Contains1AGC-kinaseC-terminaldomain.&Contains1PHdomain.&Contains1proteinkinasedomain.
      MolecularWeight60kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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