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Millipore/05-716 | Anti-Myosin Heavy Chain Antibody, clone A4.1025/05-716/200 µL
  • Millipore/05-716 | Anti-Myosin Heavy Chain Antibody, clone A4.1025/05-716/200 µL

Millipore/05-716 | Anti-Myosin Heavy Chain Antibody, clone A4.1025/05-716/200 µL

價格: ￥4068.00 市場價: 6780.00
貨號: 05-716
品牌: Millipore
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    • Description
      CatalogueNumber05-716
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-MyosinHeavyChainAntibody,cloneA4.1025
      AlternateNames
      • Myosinheavychain1
      • Myosinheavychain2x
      • MyosinheavychainIIx/d
      • Myosinheavychain,skeletalmuscle,adult1
      • myosin,heavychain1,skeletalmuscle,adult
      • myosin,heavypolypeptide1,skeletalmuscle,adult
      BackgroundInformationMyosinisoneofthetwomajorcomponentsofmuscletissueresponsIBLeforcontraction.Inmusclecellsmyosinisarrangedasthickfilamentousstructuresthatarearrangedinparalleltothinfilamentousstructurescomposedofactin.MusclecontractionisactuatedbythecouplinganduncouplingofthinandthickfilamentsthroughachemicallinkagemediatedbyATP.Thecouplinganduncouplingisarrangedsuchthatthefilamentsslideinonedirectionwhencoupledandinreversedirectionwhenuncoupledorreleased.Myosinandactinalsoservetofacilitatemotilityinnon-musclecellssuchasinslimemoldswhereshorteryetsimilarfilamentousstructuresworktogether.Anincreasingnumberofdifferentmyosinsarebeingdescribed.MyosinIisalowmolecularweightmyosinfoundinprotozoaanddoesnotself-assemble.MyosinIIistheclassicformfoundinmusclecellsthatselfassembleintothickbundles.MyosinIIisamultimericproteinthatiscomposedoftwoheavychainsandtwolightchains.
      ProductInformation
      FormatAscites
      Control
      • RIPAlysatesfromC2C12cells
      PresentationUnpurifiedmouseascitesmonoclonalIgGinbuffercontaining0.07MTris-glycine,pH7.4,0.105MNaCl,0.035%sodiumazideand30%glycerol.Liquidat-20°C.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20oCfromdateofreceipt.
      Formaximumrecoveryofproduct,centrifugethevialpriortoremovingthecap.

      HandlingRecommendations:
      Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerol-containingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationAnti-MyosinHeavyChainAntibody,cloneA4.1025isanantibodyagainstMyosinHeavyChainforuseinIH&WB.
      KeyApplications
      • Immunohistochemistry
      • WesternBlotting
      ApplicationNotesImmunohistochemistry:
      Thisantibodyhasbeenreportedbyanindependentlaboratorytodetectmyosinheavychaininzebrafishembryosandskeletalmusclesectionsfixedwith4%paraformaldehydeinapreviouslot(Blagden,C.S.,1997;Cho,M.,1994).
      BIOLOGicalInformation
      ImmunogenPartiallypurifiedproteinfromadulthumanskeletalmusclemyosin.CloneA4.1025.
      CloneA4.1025
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityThisantibodyrecognizesmyosinheavychain,Mr~220kDa.Immunoblottingwithmusclecelllysateswilloftenshowseveralclosebandsfromdifferentmyosinheavychaingenes,aswellasdegradationproducts.
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Rat
      • Mouse
      • Zebrafish
      • Drosophila
      • Rabbit
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryMyosinisamajorcontractileproteinwhichconvertschemicalenergyintomechanicalenergythroughthehydrolysisofATP.Myosinisahexamericproteincomposedofapairofmyosinheavychains(MYH)andtwopairsofnonidenticallightchains.Myosinheavychainsareencodedbyamultigenefamily.Inmammalsatleast10differentmyosinheavychain(MYH)isoformshavebeendescribedfromstriated,smooth,andnonmusclecells.Theseisoformsshowexpressionthatisspatiallyandtemporallyregulatedduringdevelopment.
      GeneSymbol
      • MYH1
      • MyHC-2X/D
      • MGC133384
      • MYHa
      • MyHC-IIx/d
      • MyHC-2x
      • Myosin-1
      • MYHSA1
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P12882#Musclecontraction.
      SIZE:1939aminoacids;223115Da
      SUBUNIT:Musclemyosinisahexamericproteinthatconsistsof2heavychainsubunits(MHC),2alkalilightchainsubunits(MLC)and2regulatorylightchainsubunits(MLC-2).
      SUBCELLULARLOCATION:Cytoplasm,myofibril.Note=Thickfilamentsofthemyofibrils.
      DOMAIN:SwissProt:P12882Therodliketailsequenceishighlyrepetitive,showingcyclesofa28-residuerepeatpatterncomposedof4heptapeptides,characteristicforalpha-helicalcoiledcoils.
      SIMILARITY:Contains1IQdomain.&Contains1myosinhead-likedomain.
      MISCELLANEOUS:Eachmyosinheavychaincanbesplitinto1lightmeromyosin(LMM)and1heavymeromyosin(HMM).Itcanlaterbesplitfurtherinto2globularsubfragments(S1)and1rod-shapedsubfragment(S2).
      MolecularWeight~220kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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