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Millipore/05-667 | Anti-phospho-CREB (Ser133) Antibody, clone 10E9/05-667/100 µg
  • Millipore/05-667 | Anti-phospho-CREB (Ser133) Antibody, clone 10E9/05-667/100 µg

Millipore/05-667 | Anti-phospho-CREB (Ser133) Antibody, clone 10E9/05-667/100 µg

價格: ¥5052.00 市場價: 8420.00

貨號: 05-667
品牌: Millipore
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    • Description
      CatalogueNumber05-667
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-phospho-CREB(Ser133)Antibody,clone10E9
      AlternateNames
      • activetranscriptionfactorCREB
      • cAMPresponsiveelementbindingprotein1
      • cAMP-responseelement-bindingprotein-1
      • transactivatorprotein
      BackgroundInformationCREBisabetaZIPtranscriptionfactorthatactivatestargetgenesthroughcAMPresponseelements.CREBisabletomediatesignalsfromnumerousphysiologicalstimuli,resultinginregulationofabroadarrayofcellularresponses.WhileCREBisexpressedinnumeroustissues,itplaysalargeregulatoryroleinthenervoussystem.CREBisbelievedtoplayakeyroleinpromotingneuronalsurvival,precursorproliferation,neuriteoutgrowthandneuronaldifferentiationincertainneuronalpopulations.Additionally,CREBsignalingisinvolvedinlearningandmemoryinseveralorganisms.CREBisabletoselectivelyactivatenumerousdownstreamgenesthroughinteractionswithdifferentdimerizationpartners.CREBisactivatedbyphosphorylationatSer133byvarioussignalingpathwaysincludingErk,Ca2+andstresssignaling.SomeofthekinasesinvolvedinphosphorylatingCREBatSer133arep90RSK,MSK,CaMKIVandMAPKAPK-2.
      ProductInformation
      FormatPurified
      Control
      • Forskolin-treatedPC12celllysate
      PresentationPurifiedmousemonoclonalIgG1κfromserum-freecellculturesupernatant,lyophilizedfrom1mLofPBS,0.09%sodiumazide,PEG,andsucrose.Lyophilized.
      StorageandShippingInformation
      StorageConditionsLyophilized:Stablefor1yearat-20oCfromdateofreceipt.
      Rehydrated:Stablefor3monthsat2-8oC.
      Aliquotsolutiontostorefrozenandavoidrepeatedfreezethawcycles.
      Applications
      ApplicationAnti-phospho-CREB(Ser133)Antibody,clone10E9isaMouseMonoclonalAntibodyfordetectionofphospho-CREB(Ser133)alsoknownasactivetranscriptionfactorCREB&hasbeenvalidatedinWB,ELISA.
      KeyApplications
      • WesternBlotting
      • ELISA
      ApplicationNotesELISA:Recommendedat0.05μg/mLbyanindependentlaboratory.
      BIOLOGicalInformation
      ImmunogenKLH-conjugated,syntheticpeptide(REILSRRP(pS)YR)correspondingtoaminoacids125-135ofhumanCREB.Theimmunizingsequenceisidenticalinhumanandmouse.Clone10E9.
      Cloneclone10E9
      HostMouse
      SpecificityRecognizesphosphorylatedCREBatMr46kDa.Mayalsorecognizep30andp38proteins,whichlikelycorrespondtophosphorylatedCREMandATF-1respectively,sincethetwoproteinssharesignificanthomologywiththephosphorylatedCREBimmunizingpeptide
      IsotypeIgG1κ
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      • Canine
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesatranscriptionfactorthatisamemberoftheleucinezipperfamilyofDNAbindingproteins.ThisproteinbindsasahomodimertothecAMP-responsiveelement,anoctamericpalindrome.Theproteinisphosphorylatedbyseveralproteinkinases,andinducestranscriptionofgenesinresponsetohormonalstimulationofthecAMPpathway.Alternatesplicingofthisgeneresultsintwotranscriptvariantsencodingdifferentisoforms.
      GeneSymbol
      • CREB1
      • CREB
      • MGC9284
      Modifications
      • Phosphorylation
      PurificationMethodThiophilicadsoptionandsizeexclusionchromatography
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P16220#ThisproteinbindsthecAMPresponseelement(CRE),asequencepresentinmanyviralandcellularpromoters.CREBstimulatestranscriptiononbindingtotheCRE.
      SIZE:341aminoacids;36688Da
      SUBUNIT:BindsDNAasadimer.InteractsPPRC1andwithHTLV-1Tax-1.
      SUBCELLULARLOCATION:Nucleus.
      PTM:Stimulatedbyphosphorylation.PhosphorylationofbothSer-133andSer-142intheSCNregulatestheactivityofCREBandparticipatesincircADIanrhythmgeneration.PhosphorylationofSer-133allowsCREBBPandEP300binding(Bysimilarity).PhosphorylateduponDNAdamage,probablybyATMorATR.
      SIMILARITY:SwissProt:P16220##BelongstothebZIPfamily.&Contains1bZIPdomain.&Contains1KID(kinase-inducIBLe)domain.
      MolecularWeight46kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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