Immunogen | KLH-conjugated,syntheticpeptide(REILSRRP(pS)YR)correspondingtoaminoacids125-135ofhumanCREB.Theimmunizingsequenceisidenticalinhumanandmouse.Clone10E9. |
Clone | clone10E9 |
Host | Mouse |
Specificity | RecognizesphosphorylatedCREBatMr46kDa.Mayalsorecognizep30andp38proteins,whichlikelycorrespondtophosphorylatedCREMandATF-1respectively,sincethetwoproteinssharesignificanthomologywiththephosphorylatedCREBimmunizingpeptide |
Isotype | IgG1κ |
SpeciesReactivity | |
AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | ThisgeneencodesatranscriptionfactorthatisamemberoftheleucinezipperfamilyofDNAbindingproteins.ThisproteinbindsasahomodimertothecAMP-responsiveelement,anoctamericpalindrome.Theproteinisphosphorylatedbyseveralproteinkinases,andinducestranscriptionofgenesinresponsetohormonalstimulationofthecAMPpathway.Alternatesplicingofthisgeneresultsintwotranscriptvariantsencodingdifferentisoforms. |
GeneSymbol | |
Modifications | |
PurificationMethod | Thiophilicadsoptionandsizeexclusionchromatography |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P16220#ThisproteinbindsthecAMPresponseelement(CRE),asequencepresentinmanyviralandcellularpromoters.CREBstimulatestranscriptiononbindingtotheCRE. SIZE:341aminoacids;36688Da SUBUNIT:BindsDNAasadimer.InteractsPPRC1andwithHTLV-1Tax-1. SUBCELLULARLOCATION:Nucleus. PTM:Stimulatedbyphosphorylation.PhosphorylationofbothSer-133andSer-142intheSCNregulatestheactivityofCREBandparticipatesincircADIanrhythmgeneration.PhosphorylationofSer-133allowsCREBBPandEP300binding(Bysimilarity).PhosphorylateduponDNAdamage,probablybyATMorATR. SIMILARITY:SwissProt:P16220##BelongstothebZIPfamily.&Contains1bZIPdomain.&Contains1KID(kinase-inducIBLe)domain. |
MolecularWeight | 46kDa |