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Millipore/05-1117 | Anti-VEGF Antibody, clone VG1/05-1117/100 µL
  • Millipore/05-1117 | Anti-VEGF Antibody, clone VG1/05-1117/100 µL

Millipore/05-1117 | Anti-VEGF Antibody, clone VG1/05-1117/100 µL

價格: ¥3576.00 市場價: 5960.00

貨號: 05-1117
品牌: Millipore
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    • Description
      CatalogueNumber05-1117
      ReplacesMAB3734
      DescriptionAnti-VEGFAntibody,cloneVG1
      AlternateNames
      • VascularpermeABIlityfactor
      • vascularendothelialgrowthfactor
      • vascularendothelialgrowthfactorA
      • vascularendothelialgrowthfactorisoformVEGF165
      BackgroundInformationVEGF(Vascularendothelialgrowthfactor,VEGFA,VEGF-A),adimericligand,isamongthemostpotentangiogenicmitogens.VEGFissecretedbytumorcellsandothercellsexposedtohypoxia.VEGFisahighlyspecificmitogenforvascularendothelialcells.SevenVEGFisoforms(a,b,c,d,e,f,g)aregeneratedasaresultofalternativesplicingfromasingleVEGFgene.ThemostcommonlystudiedisoformsareVEGF121,VEGF165,andVEGF189(f,dandb,respectively)AllsevenisoformsdifferintheirmolecularmassandinBIOLOGicalpropertiessuchastheirabilitytobindtocell-surfaceheparan-sulfateproteoglycans.TheexpressionofVEGFispotentiatedandissecretedbytumorcellsinresponsetohypoxia,byactivatedoncogenes,andbyavarietyofcytokines.
      ProductInformation
      FormatAscites
      Control
      • NIH/3T3celllysate
      PresentationUnpurifiedmousemonoclonalIgG1κinascitesfluidcontainingnopreservatives.
      StorageandShippingInformation
      StorageConditionsStablefor12monthsat-20°Cfromdateofreceipt.
      Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationDetectVEGFusingthisAnti-VEGFAntibody,cloneVG1validatedforuseinWB.
      KeyApplications
      • WesternBlotting
      BiologicalInformation
      ImmunogenRecombinanthumanVEGF189aaisoform
      CloneVG1
      HostMouse
      SpecificityDetectsthe121,165,and189aminoacidisoformsofVEGF.
      IsotypeIgG1κ
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneproductisamemberofthePDGF/VEGFgrowthfactorfamily.Itisamitogenthatspecificallyactsonendothelialcellsandhasvariouseffects,includingmediatingincreasedvascularpermeability,inducingangiogenesis,vasculogenesis,endothelialcellgrowth,promotingcellmigration,andinhibitingapoptosis.Alternativelysplicedtranscriptvariantsencodingdifferentisoformshavebeenfoundforthisgene.Also,alternativetranslationinitiationfromnon-AUG(CUG)andAUGstartsitesinsometranscriptvariants,giverisetoadditionalisoforms.
      GeneSymbol
      • MGC70609
      • VPF
      • VEGF-A
      • VEGF
      • MVCD1
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:Growthfactoractiveinangiogenesis,vasculogenesisandendothelialcellgrowth.Inducesendothelialcellproliferation,promotescellmigration,inhibitsapoptosis,andinducespermeabilizationofbloodvessels.BindstotheVEGFR1/Flt-1andVEGFR2/Kdrreceptors,heparansulfateandheparin.Neuropilin-1bindsisoformsVEGF-165andVEGF-145.
      SUBUNIT:Homodimer;disulfide-linked.AlsofoundasheterodimerwithPlGF(Bysimilarity).
      SUBCELLULARLOCATION:Secreted.Note=VEGF121isacidicandfreelysecreted.VEGF165ismorebasic,hasheparin-bindingpropertiesand,althoughasignicantproportionremainscell-associated,mostisfreelysecreted.VEGF189isverybasic,itiscell-associatedaftersecretionandisboundavidlybyheparinandtheextracellularmatrix,althoughitmaybereleasedasasolubleformbyheparin,heparinaseorplasmin.
      ALTERNATIVEPRODUCTS:10namedisoforms[FASTA]producedbyalternativesplicing.
      MolecularWeightApprox.12-28kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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