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Millipore/05-104 | Anti-EGFR Antibody, clone LA22/05-104/250 µg
  • Millipore/05-104 | Anti-EGFR Antibody, clone LA22/05-104/250 µg

Millipore/05-104 | Anti-EGFR Antibody, clone LA22/05-104/250 µg

價(jià)格: ￥3576.00 市場(chǎng)價(jià): 5960.00
貨號(hào): 05-104
品牌: Millipore
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    • Description
      CatalogueNumber05-104
      Replaces04-337;04-338
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-EGFRAntibody,cloneLA22
      AlternateNames
      • Receptortyrosine-proteinkinaseErbB-1
      • avianerythroblasticleukemiaviral(v-erb-b)oncogenehomolog
      • cellgrowthinhibitingprotein40
      • cellproliferation-inducingprotein61
      • epidermalgrowthfactorreceptor
      • epidermalgrowthfactorreceptor(avianerythroblasticleukemiaviral(v-erb-b)oncogenehomolog)
      • epidermalgrowthfactorreceptor(erythroblasticleukemiaviral(v-erb-b)oncogenehomolog
      • avian)
      BackgroundInformationProteinkinasesareenzymesthattransferaphosphategroupfromaphosphatedonorontoanacceptoraminoacidinasubstrateprotein.Bythisbasicmechanism,proteinkinasesmediatemostofthesignaltransductionineukaryoticcells,regulatingcellularmetabolism,transcription,cellcycleprogression,cytoskeletalrearrangementandcellmovement,apoptosis,anddifferentiation.Theproteinkinasefamilyisoneofthelargestfamiliesofproteinsineukaryotes,classifiedin8majorgroupsbasedonsequencecomparisonoftheirtyrosine(PTK)orserine/threonine(STK)kinasecatalyticdomains.EpidermalGrowthfactorreceptor(EGFR)istheprototypememberofthetype1receptortyrosinekinases.EGFRoverexpressionintumorsindicatespoorprognosisandisobservedintumorsoftheheadandneck,brain,bladder,stomach,breast,lung,endometrium,cervix,vulva,ovary,esophagus,stomachandinsquamouscellcarcinoma.
      ProductInformation
      FormatPurified
      Control
      • IncludedPositiveAntigenControl:Catalog#12-301,non-stimulatedA431celllysate.Add2.5μLof2-mercaptoethanol/100μLoflysateandboilfor5minutestoreducethepreparation.Load20μgofreducedlysateperlaneforminigels.
      PresentationPurifiedmousemonoclonalIgG2ainstoragebuffer(0.1MTris-glycine,pH7.4,0.15MNaCl,0.05%sodiumazide).Frozensolution.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20oCfromdateofreceipt.
      HandlingRecommendations:Uponreceiptandpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationAnti-EGFRAntibody,cloneLA22isanantibodyagainstEGFRforuseinIC,IP&WB.
      KeyApplications
      • Immunocytochemistry
      • Immunoprecipitation
      • WesternBlotting
      ApplicationNotesImmunoprecipitation:
      4μgofapreviouslotimmunoprecipitatedEGFreceptorfrom500μgofA431RIPAlysate.

      Immunocytochemistry:
      5μg/mLofpreviouslotsoftheantibodyshowedpositiveimmunostainingforEGFreceptorinA431cellsfixedwith4%paraformaldehydefollowedby100%methanol.
      BIOLOGicalInformation
      ImmunogenA431membraneproteins
      CloneLA22
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityRecognizesthesequenceofAla351-Asp364ofhumanEGFreceptor,Mr180kDa,(basedonnumberingwithoutthesignalpeptide)(Wuetal.,1989).
      IsotypeIgG2a
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheproteinencodedbythisgeneisatransmembraneglycoproteinthatisamemberoftheproteinkinasesuperfamily.Thisproteinisareceptorformembersoftheepidermalgrowthfactorfamily.EGFRisacellsurfaceproteinthatbindstoepidermalgrowthfactor.Bindingoftheproteintoaligandinducesreceptordimerizationandtyrosineautophosphorylationandleadstocellproliferation.Mutationsinthisgeneareassociatedwithlungcancer.[providedbyRefSeq].
      GeneSymbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      PurificationMethodProteinGPurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P00533#Isoform2/truncatedisoformmayactasanantagoNIST.
      SIZE:1210aminoacids;134277Da
      SUBUNIT:BindsRIPK1.CBLinteractswiththeautophosphorylatedC-terminaltailoftheEGFreceptor.PartofacomplexwithERBB2andeitherPIK3C2AorPIK3C2B.TheautophosphorylatedforminteractswithPIK3C2B,maybeindirectly.InteractswithPELP1.
      SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein.&Isoform2:Secreted.
      TISSUESPECIFICITY:Expressedinplacenta.Isoform2isalsoexpressedinovariancancers.
      PTM:PhosphorylationofSer-695ispartialandoccursonlyifThr-693isphosphorylated.&MonoubiquitinatedandpolyubiquitinateduponEGFstimulation;whichdoesnotaffecttyrosinekinaseactivityorsignalingcapacitybutmayplayaroleinlysosomaltargeting.Polyubiquitinlinkageismainlythrough"Lys-63",butlinkagethrough"Lys-48","Lys-11"and"Lys-29"alsooccur.DISEASE:SwissProt:P00533#DefectsinEGFRareassociatedwithlungcancer[MIM:211980].
      SIMILARITY:SwissProt:P00533##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.EGFreceptorsubfamily.&Contains1proteinkinasedomain.
      MISCELLANEOUS:BindingofEGFtothereceptorleadstodimerization,internalizationoftheEGF-receptorcomplex,inductionofthetyrosinekinaseactivity,stimulationofcellDNAsynthesis,andcellproliferation.
      MolecularWeight180kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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