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Millipore/05-100 | Anti-HDAC1 Antibody, clone 2E10/05-100/100 µL
  • Millipore/05-100 | Anti-HDAC1 Antibody, clone 2E10/05-100/100 µL

Millipore/05-100 | Anti-HDAC1 Antibody, clone 2E10/05-100/100 µL

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貨號: 05-100
品牌: Millipore
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    • Description
      CatalogueNumber05-100
      Replaces05-614
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-HDAC1Antibody,clone2E10
      ProductInformation
      FormatCultureSupernatant
      Presentation100μlofculturesupernatantcontaining0.05%sodiumazide.Frozenat-20°C.
      StorageandShippingInformation
      StorageConditions2yearsat-20°Cfromdateofshipment.
      Applications
      ApplicationDetectHDAC1withAnti-HDAC1Antibody,clone2E10(MouseMonoclonalAntibody),thathasbeenshowntoworkinIP&WB.
      KeyApplications
      • Immunoprecipitation
      • WesternBlotting
      BIOLOGicalInformation
      ImmunogenKLH-conjugated,syntheticpeptide(C-EEKPEAKGVKEEVKLA)correspondingtoaminoacids467-482ofmouseHDAC1.Theimmunizingsequenceisidenticalinhuman.
      Clone2E10
      HostMouse
      SpecificityHDAC-1
      IsotypeIgG1
      SpeciesReactivity
      • Human
      • Mouse
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistoneacetylationanddeacetylation,catalyzedbymultisubunitcomplexes,playakeyroleintheregulationofeukaryoticgeneexpression.Theproteinencodedbythisgenebelongstothehistonedeacetylase/acuc/aphafamilyandisacomponentofthehistonedeacetylasecomplex.Italsointeractswithretinoblastomatumor-suppressorproteinandthiscomplexisakeyelementinthecontrolofcellproliferationanddifferentiation.Togetherwithmetastasis-associatedprotein-2,itdeacetylatesp53andmodulatesitseffectoncellgrowthandapoptosis.
      GeneSymbol
      • HDAC1
      • RPD3L1
      • GON-10
      • DKFZp686H12203
      • HD1
      • RPD3
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q13547#ResponsIBLeforthedeacetylationoflysineresiduesontheN-terminalpartofthecorehistones(H2A,H2B,H3andH4).Histonedeacetylationgivesatagforepigeneticrepressionandplaysanimportantroleintranscriptionalregulation,cellcycleprogressionanddevelopmentalevents.Histonedeacetylasesactviatheformationoflargemultiproteincomplexes.
      SIZE:482aminoacids;55103Da
      SUBUNIT:Partofthecorehistonedeacetylase(HDAC)complexcomposedofHDAC1,HDAC2,RBBP4andRBBP7.ThecorecomplexassociateswithMTA2,MBD2,MBD3,MTA1L1,CHD3andCHD4toformthenucleosomeremodelingandhistonedeacetylation(NuRD)complex,orwithSIN3,SAP18andSAP30toformtheSIN3HDACcomplex.ComponentofaBHChistonedeacetylasecomplexthatcontainsHDAC1,HDAC2,HMG20B/BRAF35,AOF2/LSD1,RCOR1/CoRESTandPHF21A/BHC80.TheBHCcomplexmayalsocontainZMYM2,ZNF217,ZMYM3,GSE1andGTF2I.Associateswiththe9-1-1complex;interactswithHUS1.FoundinacomplexwithDNMT3AandHDAC7.InteractswithBCOR,BRMS1L,DAXX,DNMT1,EP300,HCFC1,PCAF,PHB2,MIER1,JMJD2A/JHDM3A,MINT,NRIP1,RERE,SETDB1,SUV39H1,TGIF,TGIF2,UHRF1andUHRF2.Interactswiththenon-histoneregionofH2AFY.InteractswithHDAC9.ComponentofamSin3AcorepressorcomplexthatcontainsSIN3A,SAP130,SUDS3/SAP45,ARID4B/SAP180,HDAC1andHDAC2.InteractswithBANP,CBFA2T3andJARID1B.InteractswithSAP30L.
      SUBCELLULARLOCATION:Nucleus.
      TISSUESPECIFICITY:Ubiquitous,withhigherlevelsinheart,pancreasandtestis,andlowerlevelsinkidneyandbrain.
      PTM:SumoylatedonLys-444andLys-476;whichpromotesenzymaticactivity.DesumoylatedbySENP1.&PhosphorylationonSer-421andSer-423promotesenzymaticactivityandinteractionswithNuRDandSIN3complexes.
      SIMILARITY:SwissProt:Q13547##Belongstothehistonedeacetylasefamily.Type1subfamily.
      MolecularWeight60kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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