Information ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals.Usage Physical State: Freeze-dried solid Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Store at 2-8°C – do not freeze. Prepare working dilution on day of use. Expiration date: six months from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Purity:Based on immunoelectrophoresis at an antigen concentration of 20 mg/ml, the pattern of precipitation against rabbit anti-donkey whole serum is the same as that against goat anti-horse IgG, F(ab")2 fragment specific.No precipitin line was detected against rabbit anti-donkey IgG, Fc fragment specific. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:0.05% Sodium Azide Conjugate Allophycocyanin (APC)
Amax: 650Emax: 660nmAllophycocyanin (APC) is among several kinds of light-harvesting phycobiliproteins found in red, blue-green, and cryptomonad algae. APC is isolated from the blue-green alga Spirulina, and is chemically cross-linked for stability. After phycobiliproteins are conjugated to secondary antibodies, there is little fluorescence quenching, which results in conjugates of high specific fluorescence compared with conventional fluorophore-antibody conjugates. APC can be excited by light over a wide range of the visible spectrum, is highly water soluble, has a relatively low isoelectric point, and lacks potentially sticky carbohydrates.It should be noted that the relatively high molecular weight of APC may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.
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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
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